Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia.
Immunomodulatory activity of polysaccharides isolated from Alchornea cordifolia.
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DOI：
10.1016/j.jep.2012.12.037
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发表时间：
2013-03-07
影响因子：
5.4
通讯作者：
Quinn, Mark T.
中科院分区：
文献类型：
--
作者：
Kouakou, Koffi;Schepetkin, Igor A.;Yapi, Ahoua;Kirpotina, Liliya N.;Jutila, Mark A.;Quinn, Mark T.
几个世纪以来，Alchornea属不同物种的叶子提取物一直用于治疗热带非洲的各种医学问题。然而，鲜为人知的是，高分子量的活性成分赋予这些提取物的治疗特性。本研究的目的是评估从Alchornea cordifolia叶中分离的多糖的免疫调节活性。从紫云英叶中提取水溶性多糖。用DEAE-cellulose、Diaion HP-20和体积排阻色谱法对cordifolia进行提取和分级。进行了分子量、糖分析和馏分的其他物理和化学表征。通过测定多糖组分诱导单核细胞/巨噬细胞一氧化氮（NO）和细胞因子产生的能力来评价多糖组分的免疫调节活性。丝裂原活化蛋白激酶（MAPK）的活化也使用磷酸化MAPK阵列进行了评估。在THP 1-Blue单核细胞中使用碱性磷酸酶报告基因测定法测定核因子κB（NF-κB）的活化。A. cordifolia分离。含有II型阿拉伯半乳聚糖的馏分具有强效免疫调节活性。特别地，母体级分AP-AU及其高分子量亚级分AP-AU 1（平均Mr估计为39.5kDa）诱导NO和细胞因子[白细胞介素（IL）-1β、-6、-10、肿瘤坏死因子（TNF）-α、和粒细胞巨噬细胞集落刺激因子（GM-CSF）]在人外周血单核细胞和人和鼠单核细胞中的作用/体外巨噬细胞细胞系。此外，用AP-AU 1处理诱导Akt 2、p38δ/p38γ、p70 S6 K1、RSK 2和mTOR的磷酸化，以及NF-κB转录活性的刺激。我们的研究结果提供了一个分子基础来解释一部分的水提取物从A有益的治疗特性。非洲传统民间医药中的心叶。
Extracts of leaves from different species of the genus Alchornea have been used for centuries to treat a variety of medicinal problems in tropical Africa. However, little is known about the high-molecular weight active components conferring therapeutic properties to these extracts. The aim of this study was to evaluate the immunomodulatory activity of polysaccharides isolated from the leaves of Alchornea cordifolia. Water-soluble polysaccharides from leaves of A. cordifolia were extracted and fractionated by DEAE-cellulose, Diaion HP-20, and size-exclusion chromatography. Molecular weight, sugar analysis, and other physical and chemical characterization of the fractions were performed. Immunomodulatory activity of the polysaccharide fractions was evaluated by determining their ability to induce monocyte/macrophage nitric oxide (NO) and cytokine production. Activation of mitogen activated protein kinases (MAPK) was also assessed using a phospho-MAPK array. Activation of nuclear factor κB (NF-κB) was measured using an alkaline phosphatase reporter gene assay in THP1-Blue monocytic cells. Six polysaccharide fractions from A. cordifolia were isolated. Fractions containing type II arabinogalactan had potent immunomodulatory activity. Particularly, the parent fraction AP-AU and its high-molecular weight sub-fraction AP-AU1 (average Mr was estimated to be 39.5 kDa) induced production of NO and cytokines [interleukin (IL)-1β, -6, -10, tumor necrosis factor (TNF)-α, and granulocyte macrophage-colony stimulating factor (GM-CSF)] in human peripheral blood mononuclear cells and human and murine monocyte/macrophages cell lines in vitro. Furthermore, treatment with AP-AU1 induced phosphorylation of Akt2, p38δ/p38γ, p70S6K1, RSK2, and mTOR, as well as stimulation of NF-κB transcriptional activity. Our results provide a molecular basis to explain a portion of the beneficial therapeutic properties of water extracts from A. cordifolia leaves in traditional folk medicine of Africa.
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影响因子：
3.7
作者：
Holderness J;Schepetkin IA;Freedman B;Kirpotina LN;Quinn MT;Hedges JF;Jutila MA
通讯作者：
Jutila MA
影响因子：
5.4
作者：
Mavar-Manga, H.;Haddad, M.;Quetin-Leclercq, J.
通讯作者：
Quetin-Leclercq, J.
影响因子：
5.4
作者：
Mesia, G. K.;Tona, G. L.;Vlietinck, A. J.
通讯作者：
Vlietinck, A. J.
影响因子：
3
作者：
Go, Hiroe;Hwang, Hye-Jung;Taek-Jeong Nam
通讯作者：
Taek-Jeong Nam
DOI：
10.1016/j.ijbiomac.2011.06.017
发表时间：
2011-11-01
影响因子：
8.2
作者：
Gao, Tingting;Ma, Shuai;Tao, Yanduo
通讯作者：
Tao, Yanduo