No Evidence Known Viruses Play a Role in the Pathogenesis of Onchocerciasis-Associated Epilepsy. An Explorative Metagenomic Case-Control Study.
No Evidence Known Viruses Play a Role in the Pathogenesis of Onchocerciasis-Associated Epilepsy. An Explorative Metagenomic Case-Control Study.
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DOI：
10.3390/pathogens10070787
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发表时间：
2021-06-22
期刊：
影响因子：
--
通讯作者：
Mokili JL
中科院分区：
文献类型：
--
作者：
Roach M;Cantu A;Vieri MK;Cotten M;Kellam P;Phan M;Hoek LV;Mandro M;Tepage F;Mambandu G;Musinya G;Laudisoit A;Colebunders R;Edwards R;Mokili JL
尽管越来越多的流行病学证据表明盘尾丝虫与儿童癫痫密切相关，因此命名为盘尾丝虫病相关性癫痫（OAE），但OAE的病理生理机制仍有待阐明。2014年6月，在刚果民主共和国（DRC）下韦莱省Titule，一项病例对照研究招募了无诱因惊厥性癫痫儿童和健康对照，以确定癫痫的风险因素。使用从入组本研究的个体（16名OAE患者和9名对照）收集的样本子集，对血浆、血沉棕黄层和脑脊液（CSF）进行随机引物下一代测序。使用灵敏的计算方法分析所得序列以鉴定病毒DNA和RNA序列。在病例和对照中鉴定了环病毒科、黄病毒科、嗜肝DNA病毒科（B型肝炎病毒）、疱疹病毒科、乳头瘤病毒科、多瘤病毒科（人多瘤病毒）和Virgaviridae。不出所料，在所有病例和对照中也检测到了各种噬菌体。然而，在OAE病例中没有发现任何已鉴定的病毒序列富集，这是我们对可能在OAE病因或发病机制中起作用的药物的标准。
Despite the increasing epidemiological evidence that the Onchocerca volvulus parasite is strongly associated with epilepsy in children, hence the name onchocerciasis-associated epilepsy (OAE), the pathophysiological mechanism of OAE remains to be elucidated. In June 2014, children with unprovoked convulsive epilepsy and healthy controls were enrolled in a case control study in Titule, Bas-Uélé Province in the Democratic Republic of the Congo (DRC) to identify risk factors for epilepsy. Using a subset of samples collected from individuals enrolled in this study (16 persons with OAE and 9 controls) plasma, buffy coat, and cerebrospinal fluid (CSF) were subjected to random-primed next-generation sequencing. The resulting sequences were analyzed using sensitive computational methods to identify viral DNA and RNA sequences. Anneloviridae, Flaviviridae, Hepadnaviridae (Hepatitis B virus), Herpesviridae, Papillomaviridae, Polyomaviridae (Human polyomavirus), and Virgaviridae were identified in cases and in controls. Not unexpectedly, a variety of bacteriophages were also detected in all cases and controls. However, none of the identified viral sequences were found enriched in OAE cases, which was our criteria for agents that might play a role in the etiology or pathogenesis of OAE.
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