COR15B expression is affected by chloroplast functionality and its role in response to salt stress in Arabidopsis thaliana
COR15B expression is affected by chloroplast functionality and its role in response to salt stress in Arabidopsis thaliana
复制标题
拟南芥中 COR15B 表达受叶绿体功能及其响应盐胁迫的作用的影响
DOI：
10.1007/s10535-014-0451-4
复制
发表时间：
2014-09
影响因子：
1.5
通讯作者：
Fu YQ
中科院分区：
文献类型：
--
作者：
Liu D;Hou L;Li WC;Cheng JF;Fu YQ
冷响应基因（COR）参与植物对低温胁迫的响应。本研究从拟南芥中分离并鉴定了一个光诱导的冷响应基因COR 15 B。突变（白化病突变体seca 1，secy 1和tic 20）或氟草松（NF）处理引起的叶绿体损伤导致COR 15 B转录减少。半定量RT-PCR分析表明，COR 15 B是由盐胁迫诱导脱落酸依赖的方式。在拟南芥中过表达COR 15 B导致转基因株系比野生型对NaCl处理更敏感。然而，COR 15 B敲除并没有显着影响cor 15 b突变体对盐胁迫的敏感性。此外，我们发现，COR 15 B的同源基因COR 15 A的表达，在cor 15 b突变体植物中显着升高。所有这些结果表明，植物获得充分表达COR 15 B的能力，只有在发育功能叶绿体。拟南芥COR 15同源基因之间可能存在表达重编程和功能备份。
Cold-responsive (COR) genes participate in the response of plants to low-temperature stress. In this study, we isolated and characterized a cold-responsive and light-inducible gene COR15B from Arabidopsis thaliana. Chloroplast damage caused by mutations (albino mutants seca1, secy1, and tic20) or by a norflurazon (NF) treatment resulted in a reduction of COR15B transcription. A semi-quantitative RT-PCR analysis shows that COR15B was induced by the salt stress in an abscisic acid-dependent manner. An over-expression of COR15B in Arabidopsis resulted in transgenic lines more sensitive to the NaCl treatment than the wild type. However, COR15B knockdown did not significantly affect the sensitivity of the cor15b mutant to the salt stress. Furthermore, we found that the expression of COR15A, a homologous gene of COR15B, was significantly elevated in cor15b mutant plants. All these results suggest that plants acquire the ability to fully express COR15B only after development of functional chloroplasts. The expressional reprogramming and functional backup may exist between COR15 homologues in Arabidopsis.
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DOI：
--
发表时间：
2011
期刊：
--
影响因子：
--
作者：
Courtney A. Skalitzky;Roy J. Martin;Jessica H. Harwood;J. Beirne;B. Adamczyk;Gregory R. Heck;Kenneth Cline;Donna E. Fernandez
通讯作者：
Courtney A. Skalitzky;Roy J. Martin;Jessica H. Harwood;J. Beirne;B. Adamczyk;Gregory R. Heck;Kenneth Cline;Donna E. Fernandez
影响因子：
3.5
作者：
Leister, D
通讯作者：
Leister, D
DOI：
10.1111/j.1365-3040.2012.02522.x
发表时间：
2012-10
期刊：
Plant, cell & environment
影响因子：
--
作者：
E. Zuther;Elisa Schulz;L. Childs;D. Hincha
通讯作者：
E. Zuther;Elisa Schulz;L. Childs;D. Hincha
影响因子：
11.6
作者：
Estavillo, Gonzalo M.;Crisp, Peter A.;Pogson, Barry J.
通讯作者：
Pogson, Barry J.
影响因子：
1.5
作者：
J. A. Silveira;A. Melo;M. O. Martins;S. L. Ferreira-Silva;R. Aragão;E. N. Silva;R. A. Viégas
通讯作者：
J. A. Silveira;A. Melo;M. O. Martins;S. L. Ferreira-Silva;R. Aragão;E. N. Silva;R. A. Viégas