Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil.
Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil.
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DOI：
10.1186/s13071-015-0714-8
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发表时间：
2015-02-15
影响因子：
3.2
通讯作者：
Cacciò SM
中科院分区：
文献类型：
--
作者：
David ÉB;Guimarães S;de Oliveira AP;Goulart de Oliveira-Sequeira TC;Nogueira Bittencourt G;Moraes Nardi AR;Martins Ribolla PE;Bueno Franco RM;Branco N;Tosini F;Bella A;Pozio E;Cacciò SM
在世界范围内，几种原生动物引起人类急性或慢性胃肠炎。生活在世界发展中地区的儿童的疾病负担特别高，那里的卫生标准较低，安全用水稀缺，有利于传播。然而，无症状感染和多寄生现象在贫困环境中也很常见。在这里，我们调查了肠道原生动物在两个小渔村，波尔图说（PS）和圣玛利亚达塞拉（SM），位于沿着在圣保罗州的Tietê河，巴西。那些村庄缺乏基本的公共基础设施和服务，如道路、公共供水、电力和公共卫生服务。从PS中的88个个体和SM中的38个个体收集多个粪便样本，这些个体在采样时无症状并且最近没有腹泻病史。为了深入了解潜在的传播途径，还收集了49份犬粪便样本（38份来自PS，11份来自SM）和28份河水样本。所有样品均通过显微镜进行检测，并使用PCR对贾第鞭毛虫、芽囊原虫、脆弱双阿米巴和隐孢子虫属。通过分子生物学方法，最常见的人体寄生虫是芽囊原虫（患病率，PS为45%，SM为71%），其次是D。fragilis（13.6%）和G.隐孢子虫（PS中18.2%，SM中7.9%）;隐孢子虫属。没有被检测到。序列分析显示芽囊原虫样本之间存在较大的遗传变异，亚型（ST）1和3占主导地位，ST 4明显缺失。在G.在人类中检测到了猪链球菌样品中的组合物A和B，而在犬中发现了组合物A、C和D。最后，所有D。来自人类的fragilis样品是基因型1。一只狗被发现感染了犬隐孢子虫。河水样品为阴性的调查寄生虫。这项研究表明，来自巴西两个贫困村庄的无症状个体的肠道寄生虫携带率很高，并强调了芽囊原虫属的巨大遗传变异性。和G.尿道炎
Several species of protozoa cause acute or chronic gastroenteritis in humans, worldwide. The burden of disease is particularly high among children living in developing areas of the world, where transmission is favored by lower hygienic standards and scarce availability of safe water. However, asymptomatic infection and polyparasitism are also commonly observed in poor settings. Here, we investigated the prevalence of intestinal protozoa in two small fishing villages, Porto Said (PS) and Santa Maria da Serra (SM), situated along the river Tietê in the State of São Paolo, Brazil. The villages lack basic public infrastructure and services, such as roads, public water supply, electricity and public health services. Multiple fecal samples were collected from 88 individuals in PS and from 38 individuals in SM, who were asymptomatic at the time of sampling and had no recent history of diarrheal disease. To gain insights into potential transmission routes, 49 dog fecal samples (38 from PS and 11 from SM) and 28 river water samples were also collected. All samples were tested by microscopy and PCR was used to genotype Giardia duodenalis, Blastocystis sp., Dientamoeba fragilis and Cryptosporidium spp. By molecular methods, the most common human parasite was Blastocystis sp. (prevalence, 45% in PS and 71% in SM), followed by D. fragilis (13.6% in PS, and 18.4% in SM) and G. duodenalis (18.2% in PS and 7.9% in SM); Cryptosporidium spp. were not detected. Sequence analysis revealed large genetic variation among Blastocystis samples, with subtypes (STs) 1 and 3 being predominant, and with the notable absence of ST4. Among G. duodenalis samples, assemblages A and B were detected in humans, whereas assemblages A, C and D were found in dogs. Finally, all D. fragilis samples from humans were genotype 1. A single dog was found infected with Cryptosporidium canis. River water samples were negative for the investigated parasites. This study showed a high carriage of intestinal parasites in asymptomatic individuals from two poor Brazilian villages, and highlighted a large genetic variability of Blastocystis spp. and G. duodenalis.
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影响因子：
2.7
作者：
Alfellani, Mohammed A.;Stensvold, C. Rune;Clark, C. Graham
通讯作者：
Clark, C. Graham
影响因子：
4.4
作者：
Ryan, U;Xiao, LH;Pavlasek, I
通讯作者：
Pavlasek, I
DOI：
10.1590/s0036-46652001000200011
发表时间：
2001-03-01
影响因子：
1.9
作者：
Franco, Regina Maura Bueno;Rocha-Eberhardt, Rosangela;Cantusio Neto, Romeu
通讯作者：
Cantusio Neto, Romeu
影响因子：
3.4
作者：
Nobre, Luciana N.;Silva, Renata V.;Franceschini, Sylvia C. C.
通讯作者：
Franceschini, Sylvia C. C.
影响因子：
5.7
作者：
Harhay MO;Horton J;Olliaro PL
通讯作者：
Olliaro PL