Ion channel activities in the Escherichia coli outer membrane.
Ion channel activities in the Escherichia coli outer membrane.
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大肠杆菌外膜中的离子通道活动。
DOI：
10.1016/0005-2736(90)90214-9
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kung,C
中科院分区：
文献类型：
--
作者：
Buechner,M;Delcour,AH;Martinac,B;Adler,J;Kung,C
通过膜片钳技术检测大肠杆菌的电特性。巨细胞或巨原生质球是通过五种不同的方法产生的。通过电子显微照相和其他标准，我们确定这些斑块很可能来自外膜。我们定期观察通过该膜中至少两种类型通道的电流。第一个电流是机械敏感且电压依赖性的，并且可以在已知主要孔蛋白（ompF、ompC、phoE、lamB）的单基因突变体中观察到；该通道可能代表小孔蛋白或一类新的外膜蛋白。正在探索第二个电压敏感通道与一种已知外膜蛋白的可能同一性。这些斑块上一致形成的高电阻密封以及门控离子通道的存在表明，外膜的大多数孔并不像通常所认为的那样静态打开，而是在静止时关闭，并且可能通过生理刺激打开。
The electrical properties ofEscherichia colicells were examined by the patch-clamp technique. Giant cells or giant spheroplasts were generated by five different methods. By electron micrographic and other criteria we determined that the patches are most likely from the outer membrane. We regularly observed currents through at least two types of channels in this membrane. The first current is mechanosensitive and voltage-dependent, and can be observed in single gene mutants of the known major porins (ompF, ompC, phoE, lamB); this channel may represent a minor porin or a new class of outer membrane protein. The possible identity of the second, voltage-sensitive channel with one of the known outer membrane proteins is being explored. The high-resistance seals consistently formed on these patches and the presence of gated ion channels suggest that most of the pores of the outer membrane are not statically open, as commonly held, but are closed at rest and may be openable by physiological stimuli.
影响因子：
4.1
作者：
KONTOS, HA;WEI, EP
通讯作者：
WEI, EP
影响因子：
20.1
作者：
LEFFLER, CW;BUSIJA, DW
通讯作者：
BUSIJA, DW
大鼠脑微血管的膜结合脂氧合酶。
DOI：
10.1016/s0006-291x(85)80156-x
发表时间：
1985
影响因子：
3.1
作者：
A. Baba;M. Kimoto;T. Tatsuno;T. Inoue;H. Iwata
通讯作者：
H. Iwata