Generation of Leydig-like cells: approaches, characterization, and challenges.
Generation of Leydig-like cells: approaches, characterization, and challenges.
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间质样细胞的产生：方法、表征和挑战
DOI：
10.4103/aja202193
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发表时间：
2022-07
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
--
作者：
睾丸间质细胞（Leydig cell，LC）产生睾酮在雄性生殖中起着至关重要的作用。LC的功能退化可导致睾酮缺乏，最终导致原发性男性性腺功能减退。移植外源性LCs的能力，产生睾酮的调节下丘脑-垂体-性腺轴可能是一个有前途的替代选择，以治疗男性原发性性腺功能减退症。最近的研究表明，有可能通过各种方法从干细胞中产生Leydig样细胞。此外，体细胞，如胚胎或成体成纤维细胞，也已成功地重编程为Leydig样细胞。在这篇综述中，我们总结了Leydig样细胞产生的最新进展，重点比较了所使用的不同方案和产生的细胞的有效性和安全性。通过进一步分析基于小信号分子和调节因子的成纤维细胞生成的Leydig样细胞的特征，我们发现，尽管该细胞可能产生睾酮，但它们与真实的LC有显著差异。对于未来的体内应用，重要的是，所产生的类固醇生成细胞不仅要评估其类固醇生成功能，还要通过蛋白质组学或转录组学工具评估其整体细胞代谢状态。
Testosterone production by Leydig cells (LCs) plays a crucial role in male reproduction. The functional degeneration of LCs can cause testosterone deficiency, ultimately resulting in primary male hypogonadism. Transplantation of exogenous LCs with the ability to produce testosterone in response to the regulation of the hypothalamus–pituitary–gonad axis could be a promising alternative option to treat male primary hypogonadism. Recent studies have shown that it is possible to generate Leydig-like cells from stem cells by various approaches. In addition, somatic cells, such as embryonic or adult fibroblasts, have also been successfully reprogrammed into Leydig-like cells. In this review, we summarized the recent advances in the generation of Leydig-like cells, with an emphasis on comparing the effectiveness and safety of different protocols used and the cells generated. By further analyzing the characteristics of Leydig-like cells generated from fibroblasts based on small signaling molecules and regulatory factors, we found that although the cells may produce testosterone, they are significantly different from real LCs. For future in vivo applications, it is important that the steroidogenic cells generated be evaluated not only for their steroidogenic functions but also for their overall cell metabolic state by proteomics or transcriptomic tools.
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影响因子：
4.1
作者：
Chen H;Ge RS;Zirkin BR
通讯作者：
Zirkin BR
影响因子：
48
作者：
Chavez A;Tuttle M;Pruitt BW;Ewen-Campen B;Chari R;Ter-Ovanesyan D;Haque SJ;Cecchi RJ;Kowal EJK;Buchthal J;Housden BE;Perrimon N;Collins JJ;Church G
通讯作者：
Church G
DOI：
10.1083/jcb.200409107
发表时间：
2004-12-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Davidoff MS;Middendorff R;Enikolopov G;Riethmacher D;Holstein AF;Müller D
通讯作者：
Müller D
影响因子：
8.8
作者：
Fang, Lyujie;Hung, Sandy S. C.;Wong, Raymond C. B.
通讯作者：
Wong, Raymond C. B.
影响因子：
8.8
作者：
Chen, Liang-Fu;Lin, Yen Ting;West, Anne E.
通讯作者：
West, Anne E.