Genetic background impacts the timing of synaptonemal complex breakdown in Drosophila melanogaster.
Genetic background impacts the timing of synaptonemal complex breakdown in Drosophila melanogaster.
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DOI：
10.1007/s00412-020-00742-9
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发表时间：
2020-12
期刊：
影响因子：
1.6
通讯作者：
Billmyre KK
中科院分区：
文献类型：
--
作者：
Wesley ER;Hawley RS;Billmyre KK
在不同遗传背景下进行的实验偶尔会出现实验重现性失败。这在果蝇中是一个严重的问题，因为没有标准的对照种群。在这里，我们说明了控制遗传背景的重要性，显示一个主要的减数分裂事件的时间，联会复合体（SC）的崩溃，在不同的遗传背景。我们评估了SC分解在三个不同的控制股票，并发现，在一个控制股票，yw; svspa-pol，SC分解早于俄勒冈州R和W1118股票。我们进一步研究了SC分解在这三个对照背景与苍蝇杂合子的无效突变c（3）G，它编码的SC的关键结构组成部分。苍蝇杂合子c（3）G显示在不同的控制背景中的SC分解的时间差异，提供了这种突变的致敏效应的证据。这些观察结果表明，在某些背景下，SC维持与c（3）G的剂量有关。最后，染色体分离不受过早SC故障中前期，与以前的研究结果，染色体分离是不依赖于全长SC中前期。因此，遗传背景是一个重要的变量，考虑在果蝇减数分裂SC的行为。本文的在线版本（10.1007/s 00412 -020-00742-9）包含补充材料，可供授权用户使用。
Experiments performed in different genetic backgrounds occasionally exhibit failure in experimental reproducibility. This is a serious issue in Drosophila where there are no standard control stocks. Here, we illustrate the importance of controlling genetic background by showing that the timing of a major meiotic event, the breakdown of the synaptonemal complex (SC), varies in different genetic backgrounds. We assessed SC breakdown in three different control stocks and found that in one control stock, y w; svspa-pol, the SC broke down earlier than in Oregon-R and w1118 stocks. We further examined SC breakdown in these three control backgrounds with flies heterozygous for a null mutation in c(3)G, which encodes a key structural component of the SC. Flies heterozygous for c(3)G displayed differences in the timing of SC breakdown in different control backgrounds, providing evidence of a sensitizing effect of this mutation. These observations suggest that SC maintenance is associated with the dosage of c(3)G in some backgrounds. Lastly, chromosome segregation was not affected by premature SC breakdown in mid-prophase, consistent with previous findings that chromosome segregation is not dependent on full-length SC in mid-prophase. Thus, genetic background is an important variable to consider with respect to SC behavior during Drosophila meiosis. The online version of this article (10.1007/s00412-020-00742-9) contains supplementary material, which is available to authorized users.
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DOI：
10.1007/978-1-59745-471-1_23
发表时间：
2009
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Doetschman, Thomas
通讯作者：
Doetschman, Thomas
影响因子：
2.6
作者：
Miller, Danny E.
通讯作者：
Miller, Danny E.
影响因子：
9.2
作者：
Merritt, Christopher;Rasoloson, Dominique;Ko, Darae;Seydoux, Geraldine
通讯作者：
Seydoux, Geraldine
影响因子：
4.5
作者：
Page SL;Khetani RS;Lake CM;Nielsen RJ;Jeffress JK;Warren WD;Bickel SE;Hawley RS
通讯作者：
Hawley RS
影响因子：
3.3
作者：
Dworkin, Ian;Kennerly, Erin;Gibson, Greg
通讯作者：
Gibson, Greg