Yeast ribosomal protein L10 helps coordinate tRNA movement through the large subunit.
Yeast ribosomal protein L10 helps coordinate tRNA movement through the large subunit.
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DOI：
10.1093/nar/gkn643
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发表时间：
2008-11
影响因子：
14.9
通讯作者：
Dinman JD
中科院分区：
文献类型：
--
作者：
Petrov AN;Meskauskas A;Roshwalb SC;Dinman JD
酵母核糖体蛋白L10（大肠杆菌L16）位于连接大亚基的许多功能区域的拓扑连接的中心。这种必需的蛋白质先前涉及核糖体生物发生，翻译保真度和mRNA稳定性等多种过程。在这里，无法维持酵母杀伤病毒被用作大亚基缺陷的代理来识别一系列L10突变体。这些映射到蛋白质的大约四个离散区域。对位于L10的n端“钩”上的突变进行了详细分析，该突变插入到25S rRNA螺旋89的凸起中，揭示了对rRNA结构的强烈影响，对应于tRNA 3 '端在延伸周期中穿过大亚基时所采取的整个路径。突变诱导的结构变化范围广泛，影响核糖体的生物发生、延伸因子结合、耐药性/超敏性、翻译保真度和病毒维持。本文讨论了L10作为核糖体信息的潜在换能器的重要性，以及它的n端结构域在易位前和易位后状态之间切换的可能作用。
Yeast ribosomal protein L10 (E. coli L16) is located at the center of a topological nexus that connects many functional regions of the large subunit. This essential protein has previously been implicated in processes as diverse as ribosome biogenesis, translational fidelity and mRNA stability. Here, the inability to maintain the yeast Killer virus was used as a proxy for large subunit defects to identify a series of L10 mutants. These mapped to roughly four discrete regions of the protein. A detailed analysis of mutants located in the N-terminal ‘hook’ of L10, which inserts into the bulge of 25S rRNA helix 89, revealed strong effects on rRNA structure corresponding to the entire path taken by the tRNA 3′ end as it moves through the large subunit during the elongation cycle. The mutant-induced structural changes are wide-ranging, affecting ribosome biogenesis, elongation factor binding, drug resistance/hypersensitivity, translational fidelity and virus maintenance. The importance of L10 as a potential transducer of information through the ribosome, and of a possible role of its N-terminal domain in switching between the pre- and post-translocational states are discussed.
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影响因子：
4.5
作者：
Bidou, L;Stahl, G;Farabaugh, PJ
通讯作者：
Farabaugh, PJ
DOI：
10.1038/10695
发表时间：
1999-07-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Agrawal, RK;Heagle, AB;Frank, J
通讯作者：
Frank, J
影响因子：
3.2
作者：
ITO, H;FUKUDA, Y;KIMURA, A
通讯作者：
KIMURA, A
影响因子：
5.4
作者：
DINMAN, JD;WICKNER, RB
通讯作者：
WICKNER, RB
影响因子：
5.6
作者：
Hansen, JL;Moore, PB;Steitz, TA
通讯作者：
Steitz, TA