Salmonella enterotoxin (Stn) regulates membrane composition and integrity.
Salmonella enterotoxin (Stn) regulates membrane composition and integrity.
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DOI：
10.1242/dmm.009324
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发表时间：
2012-07
影响因子：
4.3
通讯作者：
Kurazono H
中科院分区：
文献类型：
--
作者：
Nakano M;Yamasaki E;Ichinose A;Shimohata T;Takahashi A;Akada JK;Nakamura K;Moss J;Hirayama T;Kurazono H
沙门氏菌肠毒素（Stn）作为致病因子的作用机制是有争议的。Stn的研究表明对沙门氏菌毒力有积极和消极的影响。在这项研究中，我们试图评估Stn功能及其对沙门氏菌毒力的影响。为了研究Stn功能，我们首先使用哺乳动物细胞和鼠回肠环模型进行体外和体内分析。在这些系统中，我们没有观察到野生型沙门氏菌和β-内酰胺酶基因缺失突变体之间的毒力表型差异。接下来，我们在各种体外条件下表征了突变菌株的表型和分子特性。总细胞膜蛋白组分的蛋白质组学谱在野生型和突变体之间的不同之处在于，在突变体菌株中不存在被鉴定为OmpA的蛋白质。通过far-western blotting，发现OmpA与Stn直接相互作用。为了验证这一结果，沙门氏菌的形态进行了检查，通过透射电子显微镜，与OmpA定位进行了分析，通过免疫金标记。与野生型沙门氏菌相比，突变株具有不同的极结构和薄的周质空间; OmpA在突变株中未观察到。这些结果表明，Stn，通过调节OmpA膜定位，在膜组成和完整性的维护功能。
The mechanism of action of Salmonella enterotoxin (Stn) as a virulence factor in disease is controversial. Studies of Stn have indicated both positive and negative effects on Salmonella virulence. In this study, we attempted to evaluate Stn function and its effects on Salmonella virulence. To investigate Stn function, we first performed in vitro and in vivo analysis using mammalian cells and a murine ileal loop model. In these systems, we did not observe differences in virulence phenotypes between wild-type Salmonella and an stn gene-deleted mutant. We next characterized the phenotypes and molecular properties of the mutant strain under various in vitro conditions. The proteomic profiles of the total cell membrane protein fraction differed between wild type and mutant in that there was an absence of a protein in the mutant strain, which was identified as OmpA. By far-western blotting, OmpA was found to interact directly with Stn. To verify this result, the morphology of Salmonella was examined by transmission electron microscopy, with OmpA localization being analyzed by immunogold labeling. Compared with wild-type Salmonella, the mutant strain had a different pole structure and a thin periplasmic space; OmpA was not seen in the mutant. These results indicate that Stn, via regulation of OmpA membrane localization, functions in the maintenance of membrane composition and integrity.
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影响因子：
3.8
作者：
Chopra, AK;Huang, JH;Peterson, JW
通讯作者：
Peterson, JW
影响因子：
4
作者：
Moore, M. M.;Feist, M. D.
通讯作者：
Feist, M. D.
精氨酸的ADP-核糖基化。
影响因子：
3.5
作者：
Laing, Sabrina;Unger, Mandy;Koch-Nolte, Friedrich;Haag, Friedrich
通讯作者：
Haag, Friedrich
影响因子：
--
作者：
Kajikawa, Akinobu;Masuda, Kazuya;Igimi, Shizunobu
通讯作者：
Igimi, Shizunobu
影响因子：
3.1
作者：
MOLINA, NC;PETERSON, JW
通讯作者：
PETERSON, JW