Effects of human umbilical cord mesenchymal stem cells on inflammatory factors and miR-181a in T lymphocytes from patients with systemic lupus erythematosus
Effects of human umbilical cord mesenchymal stem cells on inflammatory factors and miR-181a in T lymphocytes from patients with systemic lupus erythematosus
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人脐带间充质干细胞对系统性红斑狼疮患者T淋巴细胞炎症因子及miR-181a的影响
DOI：
10.1177/0961203319896417
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发表时间：
2019-12
期刊：
影响因子：
2.6
通讯作者：
D Deng
中科院分区：
文献类型：
--
作者：
B Zheng;P Zhang;L Yuan;R K Chhetri;Y Guo;D Deng
目的探讨脐带间充质干细胞(UC-MSCs)对系统性红斑狼疮(SLE)患者T淋巴细胞的调节作用及其可能机制。方法选择24例SLE住院患者和28例健康体检者。用Miltenyi磁珠分选T淋巴细胞。加入重组人白介素2和CD3CD28 T细胞激活剂后，将细胞负载于预先接种UC-MSCs或未接种UC-MSCs的六孔培养板上，培养1周。收集上清液，采用酶联免疫吸附试验检测炎症因子。同时收集T淋巴细胞，用聚合酶链式反应和免疫印迹法检测SLE相关基因、蛋白和miR-181a的表达水平。结果与单独培养的T淋巴细胞相比，与UC-MSCs共培养的SLE患者T淋巴细胞中的干扰素-γ、IL-4、IL-6和IL-10水平显著降低。此外，T淋巴细胞中肿瘤坏死因子α、骨桥蛋白和核因子-kappaB的基因和蛋白表达水平显著降低，而miR-181a的表达水平显著升高(p <； 0.05或0.008)。结论UC-MSCs在体外对SLE患者的T淋巴细胞有一定的免疫调节和抑制作用，有望成为治疗SLE的有效方法。UC-MSCs可能上调miR-181a，下调炎症相关基因表达。
Objectives The present study aimed to explore the effect of umbilical cord mesenchymal stem cells (UC-MSCs) on the modulation of T lymphocytes from system lupus erythematosus (SLE) patients and the possible mechanism. Methods A total of 24 hospitalized SLE patients and 28 healthy individuals were enrolled. T lymphocytes were sorted using Miltenyi magnetic beads. After the addition of recombinant human interleukin (IL)-2 and CD3CD28 T-cell activator, cells were loaded onto six-well plates pre-inoculated or not with UC-MSCs for 1 week of culture. The supernatants were collected for testing inflammatory factors by enzyme-linked immunosorbent assay. Meanwhile, T lymphocytes were collected to assess the expression levels of genes, proteins in relation to SLE and miR-181a by polymerase chain reaction and Western blot. Results Compared with T lymphocytes cultured alone, interferon-γ, IL-4, IL-6 and IL-10 levels were significantly decreased in T lymphocytes from SLE patients co-cultured with UC-MSCs. In addition, the gene and protein expression levels of TNF alpha, osteopontin and nuclear factor-kappa B in T lymphocytes were significantly decreased, while miR-181a expression was markedly elevated (p < 0.05 or 0.008). Conclusion UC-MSCs have showed certain immunomodulatory and inhibitory effects in vitro on T lymphocytes from SLE patients, which could potentially be a beneficial treatment of the disease. UC-MSCs may up-regulate miR-181a and down-regulate inflammation-related gene expression.
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影响因子：
4.4
作者：
Kathleen L. McGuire;M. Iacobelli
通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
R. Herrera-Esparza;O. Barbosa-Cisneros;R. Villalobos-Hurtado;E. Avalos-Díaz
影响因子：
3.3
作者：
H-S Li;Y. Ning;S-B Li;P. Shao;S-J Chen;Q. Ye;X. Heng
通讯作者：
H-S Li;Y. Ning;S-B Li;P. Shao;S-J Chen;Q. Ye;X. Heng