Development of a Molecular Serotyping Scheme for Morganella morganii.
Development of a Molecular Serotyping Scheme for Morganella morganii.
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摩根氏菌分子血清分型方案的开发
DOI：
10.3389/fmicb.2021.791165
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发表时间：
2021
影响因子：
5.2
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Liu B;Guo X;Wang J;Wu P;Li S;Feng L;Liu B;Wang L
摩根摩根菌通常被视为一种人体共生菌，但也可能是一种机会致病菌，可导致多种临床感染，具有较高的发病率和死亡率。在流行病学监测和控制中，迫切需要一种高效便捷的摩根摩根菌菌株分型和鉴定方法。基于细菌表面多糖抗原（O抗原或K抗原）的血清分型是许多革兰氏阴性菌的标准分型方法。在此，通过对27株菌进行全基因组测序和比较基因组学分析，我们开发了一种基于摩根摩根菌O抗原基因簇（O - AGC）遗传变异的分子血清分型方案，并鉴定出11种不同的O - AGC类型。还通过制备抗血清和凝集实验开发了一种常规血清分型方案，结果表明它与分子血清分型方案完全一致，证实了摩根摩根菌O - AGC的变异与表型O抗原多样性相关。此外，基于每种O - AGC类型内的特定基因，开发了一种具有高特异性的基于微球的悬浮阵列（MSA）。MSA的灵敏度经测定为0.1纳克基因组DNA和10³菌落形成单位纯培养物。我们进一步分析了GenBank中可获取的104个摩根摩根菌基因组，又鉴定出另外6种新的O - AGC类型，这表明这种分子血清分型方案的扩展性良好。我们的工作为摩根摩根菌的检测和流行病学监测提供了一种重要工具，并且这种方法有可能得到广泛应用，尤其是对于那些没有高效分型方法的细菌属/种。
Morganella morganii, which is often regarded as a human commensal organism, can be an opportunistic pathogen, causing a variety of clinical infections with serious morbidity and mortality. An efficient and convenient method for subtyping and identifying M. morganii strains in epidemiological surveillance and control is urgently needed. Serotyping based on bacterial surface polysaccharide antigens (O-antigen or K-antigens) is a standard subtyping method for many gram-negative bacteria. Here, through whole genome sequencing and comparative genomics analysis of 27 strains, we developed a molecular serotyping scheme based on the genetic variation of O-antigen gene clusters (O-AGC) in M. morganii, and 11 distinct O-AGC types were identified. A conventional serotyping scheme was also developed by the production of antisera and agglutination experiments, which was shown to be perfectly consistent with the molecular serotyping scheme, confirming that the variation in M. morganii O-AGC correlated with phenotypic O-antigen diversification. Furthermore, a microsphere-based suspension array (MSA) with high specificity was developed based on the specific genes within each O-AGC type. The sensitivity of MSA was determined to be 0.1 ng of genomic DNA and 103 CFU of pure culture. We further analyzed 104 M. morganii genomes available in GenBank, and an additional six novel O-AGC types were identified, indicating that the extension of this molecular serotyping scheme is convenient. Our work provides an important tool for the detection and epidemiological surveillance of M. morganii, and this method has the potential to be widely utilized, especially for bacterial genera/species without an efficient typing approach.
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影响因子：
14.9
作者：
Mistry J;Chuguransky S;Williams L;Qureshi M;Salazar GA;Sonnhammer ELL;Tosatto SCE;Paladin L;Raj S;Richardson LJ;Finn RD;Bateman A
通讯作者：
Bateman A
影响因子：
3.7
作者：
Chaiden C;Jaresitthikunchai J;Kerdsin A;Meekhanon N;Roytrakul S;Nuanualsuwan S
通讯作者：
Nuanualsuwan S
影响因子：
5.2
作者：
Li, Yayue;Huang, Junjie;Guo, Xi
通讯作者：
Guo, Xi
影响因子：
3.6
作者：
KEENLEYSIDE, WJ;PERRY, M;WHITFIELD, C
通讯作者：
WHITFIELD, C
影响因子：
6.7
作者：
Caboni M;Pédron T;Rossi O;Goulding D;Pickard D;Citiulo F;MacLennan CA;Dougan G;Thomson NR;Saul A;Sansonetti PJ;Gerke C
通讯作者：
Gerke C