Sodium channel distribution on uninnervated and innervated embryonic skeletal myotubes.
Sodium channel distribution on uninnervated and innervated embryonic skeletal myotubes.
复制标题
无神经支配和神经支配的胚胎骨骼肌管上的钠通道分布。
DOI：
10.1002/neu.1041
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Roberts,WM
中科院分区：
文献类型：
--
作者：
Anson,BD;Roberts,WM
Acetylcholine receptor (AChR) and sodium (Na+) channel distributions within the membrane of mature vertebrate skeletal muscle fibers maximize the probability of successful neuromuscular transmission and subsequent action potential propagation. AChRs have been studied intensively as a model for understanding the development and regulation of ion channel distribution within the postsynaptic membrane. Na+channel distributions have received less attention, although there is evidence that the temporal accumulation of Na+channels at developing neuromuscular junctions (NMJs) may differ between species. Even less is known about the development of extrajunctional Na+channel distributions. To further our understanding of Na+channel distributions within junctional and extrajunctional membranes, we used a novel voltage‐clamp method and fluorescent probes to map Na+channels on embryonic chick muscle fibers as they developedin vitroandin vivo.Na+current densities on uninnervated myotubes were approximately one‐tenth the density found within extrajunctional regions of mature fibers, and showed several‐fold variations that could not be explained by a random scattering of single channels. Regions of high current density were not correlated with cellular landmarks such as AChR clusters or myonuclei. Under coculture conditions, AChRs rapidly concentrated at developing synapses, while Na+channels did not show a significant increase over the 7 day coculture period.In vivoinvestigations supported a significant temporal separation between Na+channel and AChR aggregation at the developing NMJ. These data suggest that extrajunctional Na+channels cluster together in a neuronally independent manner and concentrate at the developing avian NMJ much later than AChRs. © 2001 John Wiley & Sons, Inc. J Neurobiol 48: 42–57, 2001
登录
查看更多内容
脊椎动物运动神经元死亡和突触消除
DOI：
--
发表时间：
1987
期刊：
影响因子：
--
作者：
W. Betz
通讯作者：
W. Betz
消除禽类肌肉中快肌纤维发育过程中分布的乙酰胆碱受体簇
DOI：
10.1007/bf02456693
发表时间：
1987
期刊：
Journal of Neurocytology
影响因子：
--
作者：
W. Phillips;M. Bennett
通讯作者：
M. Bennett
培养鸡骨骼肌分化过程中钠通道的发育。
影响因子：
2.7
作者：
J. Baumgold;J. Parent;I. Spector
通讯作者：
I. Spector
影响因子：
13.9
作者：
M. Bowe;Justin R. Fallon
通讯作者：
M. Bowe;Justin R. Fallon
大鼠和人类骨骼肌的电压钳：使用改进的松散贴片技术进行测量。
DOI：
--
发表时间：
1984
期刊：
Journal of Physiology
影响因子：
--
作者：
W. Almers;W. M. Roberts;R. Ruff
通讯作者：
R. Ruff