High-throughput sequencing of cytosine methylation in plant DNA.

High-throughput sequencing of cytosine methylation in plant DNA.
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植物 DNA 中胞嘧啶甲基化的高通量测序。

DOI:
10.1186/1746-4811-9-16
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发表时间:
2013-06-07
期刊:
影响因子:
5.1
通讯作者:
Hardcastle TJ
Hardcastle TJ
中科院分区:
生物学2区
文献类型:
--
作者:
Hardcastle TJ

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胞嘧啶甲基化是植物系统中一个重要且广泛的调节因子。高通量甲基化测序的方法使甲基组的特征得到了极大的改善。在这里,我们讨论目前可用于产生和分析高通量甲基化数据测序的方法。我们还讨论了以前通过对植物甲基组测序获得的结果,并强调了这一领域仍然存在的挑战。
Cytosine methylation is a significant and widespread regulatory factor in plant systems. Methods for the high-throughput sequencing of methylation have allowed a greatly improved characterisation of the methylome. Here we discuss currently available methods for generation and analysis of high-throughput sequencing of methylation data. We also discuss the results previously acquired through sequencing plant methylomes, and highlight remaining challenges in this field.
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