Subfascial infiltration of 0.5% ropivacaine, but not 0.25% ropivacaine, exacerbates damage and inflammation in surgically incised abdominal muscles of rats.
Subfascial infiltration of 0.5% ropivacaine, but not 0.25% ropivacaine, exacerbates damage and inflammation in surgically incised abdominal muscles of rats.
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DOI：
10.1038/s41598-022-13628-w
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发表时间：
2022-06-07
影响因子：
4.6
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中科院分区：
文献类型：
--
作者：
罗哌卡因引起的手术切割肌肉的肌肉毒性尚未得到充分的研究。研究罗哌卡因浸润麻醉对剖腹大鼠切开肌肉损伤、炎症和再生的影响。罗哌卡因或生理盐水在切开的肌肉筋膜下浸润。评估疼痛相关行为和组织学肌肉损伤。CD68和MyoD免疫染色分别检测第2天和第5天巨噬细胞的浸润和第5天卫星细胞的增殖情况。术后0.25%和0.5%罗哌卡因抑制疼痛相关行为2小时。0.5%罗哌卡因单次浸润未引起未切开的完整肌肉损伤，但与生理盐水或0.25%罗哌卡因浸润大鼠相比，0.5%罗哌卡因单次和多次浸润显著增强了剖腹手术引起的肌肉损伤，cd68阳性巨噬细胞和myod阳性细胞数量增加。而生理盐水输注大鼠与0.25%罗哌卡因浸润大鼠之间无显著差异。综上所述，0.25%罗哌卡因的单次或多次筋膜下浸润不会加重手术切口肌肉的损伤和炎症，但0.5%罗哌卡因的使用可能会增加肌肉损伤，因此值得关注。
Ropivacaine-induced myotoxicity in surgically incised muscles has not been fully investigated. We evaluated the effects of infiltration anesthesia with ropivacaine on damage, inflammation and regeneration in the incised muscles of rats undergoing laparotomy. Ropivacaine or saline was infiltrated below the muscle fascia over the incised muscles. Pain-related behaviors and histological muscle damage were assessed. Macrophage infiltration at days 2 and 5 and proliferation of satellite cells at day 5 were detected by CD68 and MyoD immunostaining, respectively. Pain-related behaviors were inhibited by 0.25% and 0.5% of ropivacaine for 2 h after surgery. Single infiltration of 0.5% ropivacaine did not induce injury in intact muscles without incision, but single and repeated infiltration of 0.5% ropivacaine significantly augmented laparotomy-induced muscle injury and increased the numbers of CD68-positve macrophages and MyoD-positive cells compared to those in rats with infiltration of saline or 0.25% ropivacaine. In contrast, there were no significant differences in them between rats with saline infusion and rats with 0.25% ropivacaine infiltration. In conclusion, single or repeated subfascial infiltration of 0.25% ropivacaine can be used without exacerbating the damage and inflammation in surgically incised muscles, but the use of 0.5% ropivacaine may be a concern because of potentially increased muscle damage.
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