Ethanol elicits inhibitory effect on the growth and proliferation of tongue carcinoma cells by inducing cell cycle arrest.
Ethanol elicits inhibitory effect on the growth and proliferation of tongue carcinoma cells by inducing cell cycle arrest.
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DOI：
10.4196/kjpp.2012.16.3.153
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发表时间：
2012-06
期刊：
影响因子：
--
通讯作者：
Yoo H
中科院分区：
文献类型：
--
作者：
Le TD;Do TA;Yu R;Yoo H
通过MTT测定、caspase活性测定、Western blotting和流式细胞术评估乙醇对YD-15舌癌细胞的细胞作用。在 MTT 测定中，乙醇以剂量和时间依赖性方式抑制 YD-15 细胞的生长和增殖。将细胞暴露于酒精一定时间后，研究了低浓度乙醇浓度范围（0 至 1.5%）（不诱导 YD-15 细胞死亡的条件）下乙醇对细胞周期控制的影响。细胞周期抑制剂表达的蛋白质印迹显示，随着乙醇浓度从0%增加到1.5%，p21和p27上调，而细胞周期调节因子cdk1、cdk2和cdk4以及Cyclin A、Cyclin B1和Cyclin E1逐渐下调。流式细胞术分析细胞周期分布表明，1.5%乙醇处理24 h的YD-15细胞主要停滞在G2/M期。然而，暴露于 2.5% 或更高百分比乙醇的 YD-15 细胞中，乙醇会诱导细胞凋亡。通过Caspase活性测定或Western blotting检测裂解的PARP（Caspase-3介导的细胞凋亡的标志物）以及Caspase-3和-7的激活。我们的结果表明，乙醇在低浓度范围内通过介导细胞周期阻滞在G2/M来对YD-15舌癌细胞的生长和增殖产生抑制作用，并最终在高浓度条件下诱导细胞凋亡。
Cellular effects of ethanol in YD-15 tongue carcinoma cells were assessed by MTT assay, caspase activity assay, Western blotting and flow cytometry. Ethanol inhibited the growth and proliferation of YD-15 cells in a dose- and time-dependent manner in an MTT assay. The effects of ethanol on cell cycle control at low percent range of ethanol concentration (0 to 1.5%), the condition not inducing YD-15 cell death, was investigated after exposing cells to alcohol for a certain period of time. Western blotting on the expression of cell cycle inhibitors showed that p21 and p27 was up-regulated as ethanol concentration increases from 0 to 1.5% whilst the cell cycle regulators, cdk1, cdk2, and cdk4 as well as Cyclin A, Cyclin B1 and Cyclin E1, were gradually down-regulated. Flow cytometric analysis of cell cycle distribution revealed that YD-15 cells exposed to 1.5% ethanol for 24 h was mainly arrested at G2/M phase. However, ethanol induced apoptosis in YD-15 cells exposed to 2.5% or higher percent of ethanol. The cleaved PARP, a marker of caspase-3 mediated apoptosis, and the activation of caspase-3 and -7 were detected by caspase activity assay or Western blotting. Our results suggest that ethanol elicits inhibitory effect on the growth and proliferation of YD-15 tongue carcinoma cells by mediating cell cycle arrest at G2/M at low concentration range and ultimately induces apoptosis under the condition of high concentration.
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DOI：
10.14219/jada.archive.2001.0382
发表时间：
2001-11-01
影响因子：
3.9
作者：
Silverman, S
通讯作者：
Silverman, S
影响因子：
64.5
作者：
TOYOSHIMA, H;HUNTER, T
通讯作者：
HUNTER, T
DOI：
10.1016/s0165-3806(01)00294-2
发表时间：
2001-12-14
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Li, Z;Lin, H;Luo, J
通讯作者：
Luo, J
影响因子：
6.1
作者：
Nurmi, K.;Methuen, T.;Eklund, K. K.
通讯作者：
Eklund, K. K.
DOI：
10.1097/00000374-200203000-00009
发表时间：
2002-03-01
影响因子：
3.2
作者：
Segawa, K;Minami, K;Shigematsu, A
通讯作者：
Shigematsu, A