Trade-offs of lipid remodeling in a marine predator-prey interaction in response to phosphorus limitation.
Trade-offs of lipid remodeling in a marine predator-prey interaction in response to phosphorus limitation.
复制标题
海洋捕食者-猎物相互作用中脂质重构对磷限制的权衡。
DOI：
10.1073/pnas.2203057119
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发表时间：
2022-09-06
影响因子：
11.1
通讯作者：
Chen, Yin
中科院分区：
文献类型：
--
作者：
Guillonneau, Richard;Murphy, Andrew R. J.;Teng, Zhao-Jie;Wang, Peng;Zhang, Yu-Zhong;Scanlan, David J.;Chen, Yin
微生物的生长通常受到全球海洋中磷（P）等关键营养物质的限制。对磷限制的一个主要反应是用非磷脂质替代膜磷脂以减少其细胞磷配额。然而，脂质重塑的生物学“成本”在很大程度上是未知的。在这里，我们揭示了捕食者-猎物相互作用的权衡，从而脂质重塑的细菌猎物细胞变得更容易被原生动物捕食者消化，促进其快速增长。因此，我们强调适应非生物环境和生物相互作用（放牧）的后果之间的复杂的相互作用，这可能对微生物群落的稳定性和结构以及海洋食物网的性能产生重要影响。磷（P）是限制海洋细菌生长和初级生产的关键营养素。不出所料，海洋微生物已经进化出复杂的策略来适应P限制，其中之一涉及通过用不含P的替代脂质替换磷脂来重塑膜脂质。这一策略被世界性的海洋浮游植物和异养细菌所采用，并有助于减少细胞磷配额。然而，人们对脂质重塑的生物学后果知之甚少（如果有的话）。在这里，使用的海洋细菌Phaeopathissp。MED 193和纤毛虫Uronema marinum作为一个模型，我们试图评估细菌-原生生物相互作用的重塑效果。我们发现了一个重要的权衡要么逃避摄入或抵抗消化。因此，生长在P-充满条件下的Phaeaclum很容易被尾丝藻摄取，但不容易消化，仅支持有限的捕食者生长。相比之下，以下膜脂质重塑响应P耗竭，Phaeetrin是不太可能被捕获的尾丝，由于甘露糖基化的糖缀合物的表达减少。然而，一旦摄入，膜重塑的细胞无法防止吞噬体酸化，变得更容易消化，因此，允许纤毛虫捕食者快速生长。这种权衡之间的适应P-有限的环境和敏感性原生动物放牧表明更有效地去除低P猎物，可能有重要的影响，海洋微生物食物网的功能方面的营养能量转移和营养输出效率。
Microbial growth is often limited by key nutrients like phosphorus (P) across the global ocean. A major response to P limitation is the replacement of membrane phospholipids with non-P lipids to reduce their cellular P quota. However, the biological “costs” of lipid remodeling are largely unknown. Here, we uncover a predator–prey interaction trade-off whereby a lipid-remodeled bacterial prey cell becomes more susceptible to digestion by a protozoan predator facilitating its rapid growth. Thus, we highlight a complex interplay between adaptation to the abiotic environment and consequences for biotic interactions (grazing), which may have important implications for the stability and structuring of microbial communities and the performance of the marine food web. Phosphorus (P) is a key nutrient limiting bacterial growth and primary production in the oceans. Unsurprisingly, marine microbes have evolved sophisticated strategies to adapt to P limitation, one of which involves the remodeling of membrane lipids by replacing phospholipids with non-P-containing surrogate lipids. This strategy is adopted by both cosmopolitan marine phytoplankton and heterotrophic bacteria and serves to reduce the cellular P quota. However, little, if anything, is known of the biological consequences of lipid remodeling. Here, using the marine bacterium Phaeobacter sp. MED193 and the ciliate Uronema marinum as a model, we sought to assess the effect of remodeling on bacteria–protist interactions. We discovered an important trade-off between either escape from ingestion or resistance to digestion. Thus, Phaeobacter grown under P-replete conditions was readily ingested by Uronema, but not easily digested, supporting only limited predator growth. In contrast, following membrane lipid remodeling in response to P depletion, Phaeobacter was less likely to be captured by Uronema, thanks to the reduced expression of mannosylated glycoconjugates. However, once ingested, membrane-remodeled cells were unable to prevent phagosome acidification, became more susceptible to digestion, and, as such, allowed rapid growth of the ciliate predator. This trade-off between adapting to a P-limited environment and susceptibility to protist grazing suggests the more efficient removal of low-P prey that potentially has important implications for the functioning of the marine microbial food web in terms of trophic energy transfer and nutrient export efficiency.
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影响因子：
3.7
作者：
Friman VP;Ghoul M;Molin S;Johansen HK;Buckling A
通讯作者：
Buckling A
影响因子：
1.4
作者：
Hahn, MW;Lünsdorf, H;Janke, L
通讯作者：
Janke, L
影响因子：
28.3
作者：
Dadon-Pilosof, Ayelet;Conley, Keats R.;Yahel, Gitai
通讯作者：
Yahel, Gitai
影响因子：
4.5
作者：
Guillonneau R;Baraquet C;Molmeret M
通讯作者：
Molmeret M
DOI：
10.1590/s1517-838220090001000014
发表时间：
2009-01
期刊：
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
影响因子：
--
作者：
Martel CM
通讯作者：
Martel CM