Nitrogen ligation to manganese in the photosynthetic oxygen-evolving complex: continuous-wave and pulsed EPR studies of photosystem II particles containing nitrogen-14 or nitrogen-15
Nitrogen ligation to manganese in the photosynthetic oxygen-evolving complex: continuous-wave and pulsed EPR studies of photosystem II particles containing nitrogen-14 or nitrogen-15
复制标题
光合放氧复合物中氮与锰的连接：含氮 14 或氮 15 的光系统 II 粒子的连续波和脉冲 EPR 研究
DOI：
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
M. Klein
中科院分区：
文献类型：
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作者：
V. DeRose;V. Yachandra;A. McDermott;R. Britt;K. Sauer;M. Klein
用电子顺磁共振(EPR)和电子自旋回波包络调制(ESEEM)谱研究了氮与光系统II放氧复合体中的锰连接的可能性。从嗜热蓝藻聚球藻中分离到放氧制剂，该菌生长在以{sup 14}no{sub3{sup{-}或{sup 15}no{sup{-}}{sub3}为唯一氮源的培养基上。用连续波电子顺磁共振测量了酶的S{sub2}态的锰产生的多线电子顺磁共振信号的亚结构。用{sup 15}N取代{sup 14}N后，亚结构的峰位没有发生变化，表明这种亚结构不是由于氮配体的超精细耦合所致。为了检测具有比常规EPR方法所能观察到的更小的超精细耦合的潜在氮配体，还对多线EPR信号进行了电子自旋回波包络调制实验。对亮-暗-暗时间域ESEEM数据的傅里叶变换显示，在{sup 14}N样品中有一个位于4.8 MHz的峰，该峰在被{sup 15}N取代后消失。这为氮与析氧络合物的Mn的弱超精细耦合提供了明确的证据。我们还讨论了这种氮相互作用的起源。
The possibility of nitrogen ligation to the Mn in the oxygen-evolving complex from photosystem II was investigated with electron paramagnetic resonance (EPR) and electron spin echo envelope modulation (ESEEM) spectroscopies using {sup 14}N- and {sup 15}N-labeled preparations. Oxygen-evolving preparations were isolated from a thermophilic cyanobacterium, Synechococcus sp., grown on a medium containing either {sup 14}NO{sub 3{sup {minus}}} or {sup 15}NO{sup {minus}}{sub 3} as the sole source of nitrogen. The substructure on the multiline EPR signal, which arises from Mn in the S{sub 2} state of the enzyme, was measured with continuous-wave EPR. No changes were detected in the substructure peak positions upon substitution of {sup 15}N for {sup 14}N, indicating that this substructure is not due to superhyperfine coupling from nitrogen ligands. To detect potential nitrogen ligands with superhyperfine couplings of lesser magnitude than could be observed with conventional EPR methods, electron spin-echo envelope modulation experiments were also performed on the multiline EPR signal. The Fourier transform of the light-minus-dark time domain ESEEM data shows a peak at 4.8 MHz in {sup 14}N samples which is absent upon substitution with {sup 15}N. This gives unambiguous evidence for weak hyperfine coupling of nitrogen to the Mn of the oxygen-evolving complex. Possiblemore » origins of this nitrogen interaction are discussed.« less
蓝铜和铁硫蛋白中硫配体的氢键：通过共振拉曼光谱检测。
影响因子：
2.9
作者：
Mino,Y;Loehr,TM;Wada,K;Matsubara,H;Sanders-Loehr,J
通讯作者：
Sanders-Loehr,J
影响因子：
2.9
作者：
BABCOCK, GT;BARRY, BA;YOCUM, CF
通讯作者：
YOCUM, CF
影响因子：
2.9
作者：
DEPAULA, JC;INNES, JB;BRUDVIG, GW
通讯作者：
BRUDVIG, GW