Premetazoan genome evolution and the regulation of cell differentiation in the choanoflagellate Salpingoeca rosetta.
Premetazoan genome evolution and the regulation of cell differentiation in the choanoflagellate Salpingoeca rosetta.
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DOI：
10.1186/gb-2013-14-2-r15
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发表时间：
2013-02-18
期刊：
影响因子：
12.3
通讯作者：
King N
中科院分区：
文献类型：
--
作者：
Fairclough SR;Chen Z;Kramer E;Zeng Q;Young S;Robertson HM;Begovic E;Richter DJ;Russ C;Westbrook MJ;Manning G;Lang BF;Haas B;Nusbaum C;King N
后生动物的多细胞性源于细胞黏附、信号传导和分化机制，这些机制最初在后生动物的祖先中进化而来。为了重建后生动物祖先的基因组组成，我们对领鞭毛虫类的Salpingoeca rosetta的基因组和转录组进行了测序，它是后生动物的近亲，能形成玫瑰花结状的细胞群落。
将55Mb的Salpingoeca rosetta基因组与多种后鞭毛生物的基因组进行比较表明，在后生动物起源之前，有一段动态的基因得失时期。Salpingoeca rosetta基因组编码了此前仅在后生动物中发现的细胞黏附、神经肽和鞘糖脂代谢基因的同源物，并扩充了推断存在于后生动物和领鞭毛虫祖先中的基因库。转录组分析显示，相对于单细胞，Salpingoeca rosetta的所有四种隔蛋白在群落中都上调，这表明这些保守的胞质分裂蛋白可能在群落发育过程中调节不完全的胞质分裂。此外，后生动物和领鞭毛虫特有的基因在群落以及形成群落的单细胞中不成比例地上调。
Salpingoeca rosetta基因组序列完善了后生动物特异性基因的目录，同时也扩展了某些对后生动物生物学至关重要的基因家族的进化历史。转录组数据表明，包括隔蛋白在内的保守胞质分裂基因可能有助于Salpingoeca rosetta群落的形成，并表明群落发育的起始可能优先利用与后生动物共有的基因，而群落成熟的后期阶段可能由Salpingoeca rosetta特有的基因调控。
Metazoan multicellularity is rooted in mechanisms of cell adhesion, signaling, and differentiation that first evolved in the progenitors of metazoans. To reconstruct the genome composition of metazoan ancestors, we sequenced the genome and transcriptome of the choanoflagellate Salpingoeca rosetta, a close relative of metazoans that forms rosette-shaped colonies of cells. A comparison of the 55 Mb S. rosetta genome with genomes from diverse opisthokonts suggests that the origin of metazoans was preceded by a period of dynamic gene gain and loss. The S. rosetta genome encodes homologs of cell adhesion, neuropeptide, and glycosphingolipid metabolism genes previously found only in metazoans and expands the repertoire of genes inferred to have been present in the progenitors of metazoans and choanoflagellates. Transcriptome analysis revealed that all four S. rosetta septins are upregulated in colonies relative to single cells, suggesting that these conserved cytokinesis proteins may regulate incomplete cytokinesis during colony development. Furthermore, genes shared exclusively by metazoans and choanoflagellates were disproportionately upregulated in colonies and the single cells from which they develop. The S. rosetta genome sequence refines the catalog of metazoan-specific genes while also extending the evolutionary history of certain gene families that are central to metazoan biology. Transcriptome data suggest that conserved cytokinesis genes, including septins, may contribute to S. rosetta colony formation and indicate that the initiation of colony development may preferentially draw upon genes shared with metazoans, while later stages of colony maturation are likely regulated by genes unique to S. rosetta.
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DOI：
10.1073/pnas.0801667105
发表时间：
2008-10-28
影响因子：
11.1
作者：
Carr, M.;Leadbeater, B. S. C.;Baldauf, S. L.
通讯作者：
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影响因子：
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影响因子：
12.3
作者：
Haas, Brian J.;Salzberg, Steven L.;Zhu, Wei;Pertea, Mihaela;Allen, Jonathan E.;Orvis, Joshua;White, Owen;Buell, C. Robin;Wortman, Jennifer R.
通讯作者：
Wortman, Jennifer R.
DOI：
10.1073/pnas.0306880101
发表时间：
2004-08-10
影响因子：
11.1
作者：
Caenepeel, S;Charydczak, G;Manning, G
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Manning, G