A ratiometric bilirubin sensor based on a fluorescent gold nanocluster film with dual emissions.
A ratiometric bilirubin sensor based on a fluorescent gold nanocluster film with dual emissions.
复制标题
基于双发射荧光金纳米簇薄膜的比例胆红素传感器。
DOI：
10.1039/d0ay01781g
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发表时间：
2020-11
影响因子：
3.1
通讯作者：
Zhencheng Chen
中科院分区：
文献类型：
--
作者：
Wenxiang Xiao;Dongcan Zhi;Qiaoni Pan;Fang Zhou;Yongbo Liang;Zhencheng Chen
胆红素来源于血红蛋白代谢，是肝功能的重要生物标志物。制造了一种基于金纳米簇 (AuNC) 的比率薄膜传感器，用于高灵敏度测定游离胆红素 (fBR)。以牛血清白蛋白（BSA）为模板，通过水热法在不同pH值下制备出能发出蓝色和红色荧光的AuNCs。通过层层组装（LBL）技术将两种 AuNC 整合到单个薄膜中。获得的薄膜显示出在 372 nm 处激发的双荧光峰，分别对应于 AuNC 的蓝色（443 nm）和红色（622 nm）发射。当 fBR 与薄膜相互作用时，两个荧光峰都有不同程度的猝灭。根据两次发射的荧光强度比建立了 fBR 检测的比率法。 fBR 的线性校准曲线在 0.01-2.00 μmol L-1 的浓度范围内，检测限为 8.90 ± 0.34 nmol L-1 (S/N = 3)。该薄膜传感器对fBR表现出快速、灵敏的响应，可以检测实际样品中的fBR，并获得满意的结果。
Bilirubin originates from hemoglobin metabolism and is an important biomarker for liver function. A ratiometric film sensor based on gold nanoclusters (AuNCs) was fabricated for highly sensitive determination of free bilirubin (fBR). Using bovine serum albumin (BSA) as a template, AuNCs that can emit blue and red fluorescence were prepared by the hydrothermal method at different pH values. Two kinds of AuNCs were incorporated into a single film by the layer-by-layer assembly (LBL) technique. The obtained thin-film showed dual fluorescence peaks excited at 372 nm, corresponding to the blue (443 nm) and red (622 nm) emissions of AuNCs respectively. When fBR interacted with the film, both fluorescence peaks were quenched at different degrees. A ratiometric method for fBR detection was established based on the fluorescence intensity ratio of the two emissions. The linear calibration curve for fBR lay in the concentration range of 0.01-2.00 μmol L-1 with a detection limit of 8.90 ± 0.34 nmol L-1 (S/N = 3). The film sensor showed a quick and sensitive response to fBR and could detect fBR in real samples with satisfactory results.
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影响因子：
7.4
作者：
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通讯作者：
Aditya Sharma;C. M. Pandey;Zimple Matharu;U. Soni;S. Sapra;G. Sumana;M. Pandey;T. Chatterjee;B. Malhotra
影响因子：
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作者：
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通讯作者：
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作者：
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通讯作者：
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作者：
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通讯作者：
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影响因子：
3.8
作者：
Goncharova, Iryna;Orlov, Sergey;Urbanova, Marie
通讯作者：
Urbanova, Marie