Human growth hormone as a reporter gene in regulation studies employing transient gene expression
Human growth hormone as a reporter gene in regulation studies employing transient gene expression
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人类生长激素作为瞬时基因表达调控研究中的报告基因
DOI：
10.1128/mcb.6.9.3173-3179.1986
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发表时间：
1986
影响因子：
5.3
通讯作者：
D. Moore
中科院分区：
文献类型：
--
作者：
R. Selden;K. B. Howie;M. Rowe;H. Goodman;D. Moore
这里描述的人类生长激素（hGH）瞬时测定系统是基于hGH的表达，由细胞转染hGH融合基因。培养基中分泌hGH的水平，通过简单的放射免疫测定，与细胞质hGH mRNA的水平和转染DNA的数量成正比。该系统非常敏感，易于操作，并且与其他瞬时表达系统在质量上不同，因为培养基被检测，细胞本身不会被破坏。hGH瞬时检测系统适用于基因表达调控分析，本研究利用猴病毒40增强子对单纯疱疹病毒胸苷激酶启动子的影响和锌对小鼠金属硫蛋白- 1启动子的影响进行了研究。hGH的表达也可以与其他瞬时表达系统一起作为监测转染效率的内部控制。迄今为止测试的所有细胞类型（包括at -20、CV-1、GC、GH4、JEG、L和垂体原代细胞）都能在培养基中分泌hGH。
The human growth hormone (hGH) transient assay system described here is based on the expression of hGH directed by cells transfected with hGH fusion genes. Levels of secreted hGH in the medium, measured by a simple radioimmunoassay, are proportional to both levels of cytoplasmic hGH mRNA and the amount of transfected DNA. The system is extremely sensitive, easy to perform, and is qualitatively different from other transient expression systems in that the medium is assayed and the cells themselves are not destroyed. The hGH transient assay system is appropriate for analyses of regulation of gene expression and was utilized here to investigate the effect of the simian virus 40 enhancer on the herpes simplex virus thymidine kinase promoter and the effect of zinc on the mouse metallothionein-I promoter. The expression of hGH can also be used as an internal control to monitor transfection efficiency along with any other transient expression system. All cell types tested thus far (including AtT-20, CV-1, GC, GH4, JEG, L, and primary pituitary cells) were able to secrete hGH into the medium.
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影响因子：
56.9
作者：
EDLUND, T;WALKER, MD;RUTTER, WJ
通讯作者：
RUTTER, WJ
快速检测转导细胞中大肠杆菌黄嘌呤-鸟嘌呤磷酸核糖转移酶活性。
影响因子：
14.9
作者：
Chu,G;Berg,P
通讯作者：
Berg,P
人类生长激素 DNA 序列和 mRNA 结构：可能的选择性剪接。
影响因子：
14.9
作者：
DeNoto,FM;Moore,DD;Goodman,HM
通讯作者：
Goodman,HM
影响因子：
14.9
作者：
LOPATA, MA;CLEVELAND, DW;SOLLNERWEBB, B
通讯作者：
SOLLNERWEBB, B