Seminal plasma factors induce in vitro PRL secretion in smooth muscle cells of the human prostate.
Seminal plasma factors induce in vitro PRL secretion in smooth muscle cells of the human prostate.
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精浆因子诱导人前列腺平滑肌细胞体外分泌 PRL。
DOI：
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发表时间：
2001
影响因子：
5.8
通讯作者：
Peter Berger
中科院分区：
文献类型：
--
作者：
G. Untergasser;Holger Rumpold;Eugen Plas;Marc Witkowski;Peter Berger
除了性类固醇激素 T 之外，PRL 已被证明可以影响前列腺功能和发育。过度表达大鼠 PRL 基因的转基因小鼠的前列腺会急剧增大。在啮齿动物和男性中，PRL 会刺激上皮细胞的增殖和分泌活动。在人前列腺上皮细胞 (EC) 中观察到低浓度的人 PRL (hPRL) 和 hPRL 受体。本研究的目的是比较用含有多种前列腺因子的精浆 (SMP) 刺激后前列腺 EC 和基质平滑肌细胞 (SMC) 体外 hPRL 分泌的调节。 SMC 释放高达 1 ng hPRL/ml（即比未刺激的 SMC 和 EC 多约 500 倍）。通过高灵敏定量 RT-PCR 对 PRL mRNA 进行定量显示，hPRL 基因表达在 SMP 孵育 24 小时内增加了 5 倍。性类固醇（二氢睾酮、黄体酮、17β-雌二醇）、前列腺素（PGE-1、PGE-2）和 cAMP 刺激物质（毛喉素）与 hPRL 的诱导无关。与子宫内膜 SMC 相比，前列腺 hPRL 分泌的调节不依赖于孕酮和 cAMP。人类 SMP 的 HPLC 分析表明，需要至少两种不同蛋白质和低分子辅助因子的共同作用。我们得出的结论是，前列腺内皮细胞分泌的蛋白质与低分子量辅助因子协同作用，诱导平滑肌细胞的分化和 hPRL 释放。 SMC 衍生的 hPRL 与年龄相关的增加可能会导致前列腺良性增生的发生。
Next to the sex steroid hormone T, PRL has been shown to influence prostatic function and development. Transgenic mice overexpressing the rat PRL gene develop dramatic enlargements of the prostate gland. Proliferation and secretory activities of epithelial cells are stimulated by PRL in rodents and men. Low concentrations of human PRL (hPRL) and hPRL receptors have been observed in human prostatic epithelial cells (ECs). The aim of this study was to compare regulation of the in vitro hPRL secretion in prostatic ECs and stromal smooth muscle cells (SMCs) after stimulation with seminal plasma (SMP), containing a variety of prostatic factors. SMCs released up to 1 ng hPRL/ml (i.e., approximately 500-fold more than unstimulated SMCs and ECs). Quantification of PRL mRNA by highly sensitive quantitative RT-PCR revealed that hPRL gene expression increased 5-fold within 24 h of SMP incubation. Sex steroids (dihydrotestosterone, progesterone, 17beta-estradiol), prostaglandins (PGE-1, PGE-2), and cAMP-stimulating substances (forskolin) were not responsible for induction of hPRL. Compared with endometrial SMCs, regulation of prostatic hPRL secretion was independent of progesterone and cAMP. HPLC analysis of human SMP revealed that the common action of at least two different proteins and a low molecular cofactor is required. We concluded that prostatic ECs secrete proteins acting synergistically with low-molecular-weight cofactors to induce differentiation and hPRL release in SMCs. Age-related increases in SMC-derived hPRL might contribute to the development of benign hyperplasia of the prostate.
影响因子：
6
作者：
Leav, I;Merk, FB;Ho, SM
通讯作者：
Ho, SM
催乳素是器官培养中雄激素剥夺的大鼠背侧和外侧前列腺上皮的存活因子。
DOI：
10.1210/endo.140.11.7090
发表时间：
1999
期刊：
Endocrinology.
影响因子：
--
作者：
Ahonen,TJ;Harkonen,PL;Laine,J;Rui,H;Martikainen,PM;Nevalainen,MT
通讯作者：
Nevalainen,MT
影响因子：
4.8
作者：
G. Frank;A. Brar;M. Cedars;S. Handwerger
通讯作者：
G. Frank;A. Brar;M. Cedars;S. Handwerger