Evaluation of micro-well collector for capture and analysis of aerosolized Bacillus subtilis spores.
Evaluation of micro-well collector for capture and analysis of aerosolized Bacillus subtilis spores.
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DOI：
10.1371/journal.pone.0197783
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Novosselov I
中科院分区：
文献类型：
--
作者：
He J;Beck NK;Kossik AL;Zhang J;Seto E;Meschke JS;Novosselov I
生物气溶胶采样和鉴定对于评估和控制空气传播的病原体、过敏原和毒素至关重要。化学和生物颗粒物的现场分析可以显著降低样品保存、运输和分析的成本。由于样品在大的采集区被稀释，传统过滤器的分析是具有挑战性的。开发了一种用于收集和分析气雾剂的低成本墨盒，用于流行病学研究和个人暴露评估。该滤芯在微孔中收集气溶胶样品，从而减少了由于反弹造成的颗粒损失，并且不需要任何涂层。受限的颗粒收集区域(停留~1.4 mm)可减少后续分析的洗脱体积。使用雾化细菌芽胞(枯草芽孢杆菌)在实验室研究中验证了该药盒的性能。菌落形成单位分析用于细菌孢子计数。通过与参考过滤器进行比较来评估滤筒收集效率，并发现其与测试的流速一致。吸管洗脱样品回收率约为80%。由于采集的样品密度高，该色谱柱与现场光谱分析和样品洗脱兼容，可在10-20μ的L液体体积内进行洗脱，从而显著提高后续分析的样品浓度。
Bioaerosol sampling and identification are vital for the assessment and control of airborne pathogens, allergens, and toxins. In-situ analysis of chemical and biological particulate matter can significantly reduce the costs associated with sample preservation, transport, and analysis. The analysis of conventional filters is challenging, due to dilute samples in large collection regions. A low-cost cartridge for collection and analysis of aerosols is developed for use in epidemiological studies and personal exposure assessments. The cartridge collects aerosol samples in a micro-well which reduces particles losses due to the bounce and does not require any coating. The confined particle collection area (dwell~1.4 mm) allows reducing the elution volume for subsequent analysis. The performance of the cartridge is validated in laboratory studies using aerosolized bacterial spores (Bacillus subtilis). Colony forming unit analysis is used for bacterial spore enumeration. Cartridge collection efficiency is evaluated by comparison with the reference filters and found to be consistent with tested flow rates. Sample recovery for the pipette elution is ~80%. Due to the high density of the collected sample, the cartridge is compatible with in-situ spectroscopic analysis and sample elution into the 10–20 μl liquid volume providing a significant increase in sample concentration for subsequent analysis.
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