Dose-response effect of berberine on bile acid profile and gut microbiota in mice.
Dose-response effect of berberine on bile acid profile and gut microbiota in mice.
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小檗碱对小鼠胆汁酸谱和肠道微生物群的剂量反应效应
DOI：
10.1186/s12906-016-1367-7
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发表时间：
2016-10-18
影响因子：
--
通讯作者：
Klaassen CD
中科院分区：
文献类型：
--
作者：
Guo Y;Zhang Y;Huang W;Selwyn FP;Klaassen CD
研究背景小檗碱（BBR）是一种传统的抗微生物中草药。最近，BBR作为降低血脂、胆固醇和葡萄糖的补充剂越来越受欢迎。已知胆汁酸（BA）调节甘油三酯、胆固醇、葡萄糖和能量稳态的血液水平，并且肠道植物群在BA代谢中起重要作用。方法采用UPLC-MS/MS法测定不同剂量的BBR对雄性C57 BL/6小鼠肝脏和血清中BA浓度的影响，并检测BA相关基因的表达，以及通过RT-PCR和Quantigene2.0试剂系统定量雄性C57 BL/6小鼠末端回肠和大肠中32种最丰富的肠道细菌种类的量，结果BBR对血清中未结合BAs和总BAs含量有显著影响，但对肝脏无影响。在喂食BBR的小鼠的肝脏和血清中观察到原发性BA（βMCA、TβMCA和TUDCA）增加和继发性BA（DCA、LCA和T结合物）减少。在喂食较高剂量BBR的小鼠肝脏中，BA合成酶（Cyp 7a 1和8b 1）和摄取转运蛋白（Ntcp）的表达增加了39- 400%，而核受体和外排转运蛋白没有显著改变。结论不同剂量的BBR对BA代谢及相关基因和肠道植物群均有影响，这为BBR作用的多种途径提供了依据。
BackgroundBerberine (BBR) is a traditional antimicrobial herbal medicine. Recently, BBR has gained popularity as a supplement to lower blood lipids, cholesterol and glucose. Bile acids (BAs) are known to regulate blood levels of triglycerides, cholesterol, glucose and energy homeostasis, and gut flora play an important role in BA metabolism. However, whether BBR alters BAs metabolism or dose-response effect of BBR on gut flora is unknown.MethodsIn this study, the effects of various doses of BBR on the concentrations of BAs in liver and serum of male C57BL/6 mice were determined by UPLC-MS/MS, and the expression of BA-related genes, as well as the amount of 32 of the most abundant gut bacterial species in the terminal ileum and large intestine of male C57BL/6 mice were quantified by RT-PCR and Quantigene 2.0 Reagent System, respectively.ResultsUnconjugated BAs and total BAs were significantly altered by BBR in serum but not in liver. Increased primary BAs (βMCA, TβMCA and TUDCA) and decreased secondary BAs (DCA, LCA and the T-conjugates) were observed in livers and serum of mice fed BBR. The expression of BA-synthetic enzymes (Cyp7a1 and 8b1) and uptake transporter (Ntcp) increased 39-400 % in liver of mice fed the higher doses of BBR, whereas nuclear receptors and efflux transporters were not markedly altered. In addition,Bacteroideswere enriched in the terminal ileum and large bowel of mice treated with BBR.ConclusionThe present study indicated that various doses of BBR have effects on BA metabolism and related genes as well as intestinal flora, which provides insight into many pathways of BBR effects.
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影响因子：
5.8
作者：
Li, Yuanyuan;Jadhav, Kavita;Zhang, Yanqiao
通讯作者：
Zhang, Yanqiao
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4.1
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Jiang, Qin;Liu, Peiqing;Huang, Heqing
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Kubitz, Ralf
影响因子：
6.1
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