Transdentinal cytotoxicity of experimental adhesive systems of different hydrophilicity applied to ethanol-saturated dentin.
Transdentinal cytotoxicity of experimental adhesive systems of different hydrophilicity applied to ethanol-saturated dentin.
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DOI：
10.1016/j.dental.2013.07.006
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发表时间：
2013-09
期刊：
影响因子：
5
通讯作者：
Hebling, Josimeri
中科院分区：
文献类型：
--
作者：
Bianchi, Luciana;Dias Ribeiro, Ana Paula;de Oliveira Carrilho, Marcela Rocha;Pashley, David H.;de Souza Costa, Carlos Alberto;Hebling, Josimeri
本研究的目的是评价实验性粘附系统（EAS）的不同亲水性和牙本质饱和溶液（乙醇和水）对成牙本质细胞样细胞的跨牙本质细胞毒性。将100个0.4 mm厚的牙本质盘固定在离体牙髓腔中，并分为10组。将成牙本质细胞样细胞MDPC-23接种于盘的髓侧，孵育48小时。将亲水性增加的EASs（R2，R3，R4和R5）应用于酸蚀和水或乙醇饱和脱矿牙本质后的盘的咬合侧。RO（水和乙醇-无粘合剂）作为对照。24小时后，通过SDH酶产生（MTT测定; n=8个盘）评估细胞代谢，并通过SEM（n=2个盘）检查细胞形态。通过流式细胞术鉴定细胞死亡的类型，并在两次光活化时间（10 s或20 s）后通过红外光谱法（FTIR）测定单体转化率（%DC）。通过Kruskal-Wallis和Mann-Whitney检验（α=0.05）对数据进行统计学分析。与水饱和相比，用乙醇饱和牙本质导致R3、R4和R5的坏死细胞死亡率更高，尽管R3和R4诱导了更高的SDH产生。与10 s相比，20 s的光活化显著改善了所有EAS的%DC。对于两种光活化时间，在亲水性程度和%DC之间观察到显著的正相关性。总之，除R2外，用乙醇饱和牙本质增加了EAS的细胞毒性，如通过诱导坏死细胞死亡所表示的。
The aim of this study was to evaluate the transdentinal cytotoxicity of experimental adhesive systems (EASs) with different hydrophilicity and dentin saturation solutions (ethanol and water) on odontoblast-like cells. One hundred 0.4-mm-thick dentin discs were mounted in in vitro pulp chambers and assigned to 10 groups. Odontoblast-like cells MDPC-23 were seeded onto the pulpal side of the discs, incubated for 48h. The EASs with increasing hydrophilicity (R2, R3, R4 and R5) were applied to the occlusal side of the discs after acid etching and saturation of demineralized dentin with water or ethanol. R0 (water and ethanol- no adhesive) served as controls. After 24h, cell metabolism was evaluated by SDH enzyme production (MTT assay; n=8 discs) and cell morphology was examined by SEM (n=2 discs). The type of cell death was identified by flow cytometry and the degree of monomer conversion (%DC) was determined by infrared spectroscopy (FTIR) after two photoactivation times (10 s or 20 s). Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests (α=0.05). Dentin saturation with ethanol resulted in higher necrotic cell death ratios for R3, R4 and R5 compared with water saturation, although R3 and R4 induced higher SDH production. Photoactivation for 20 s significantly improved the %DC of all EASs compared with 10 s. A significant positive correlation was observed between the degree of hydrophilicity and %DC, for both photoactivation times. In conclusion, except for R2, dentin saturation with ethanol increased the cytotoxicity of EASs, as expressed by the induction of necrotic cell death.
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影响因子：
1.9
作者：
Carrilho, Marcela R.;Tay, Franklin R.;Pashley, David H.
通讯作者：
Pashley, David H.
影响因子：
5
作者：
About, I;Camps, J;Franquin, JC
通讯作者：
Franquin, JC
DOI：
10.1002/jbm.b.30651
发表时间：
2007-04-01
影响因子：
3.4
作者：
Alberto de Souza Costa, Carlos;Teixeira, Hilcia Mezzalira;Hebling, Josimeri
通讯作者：
Hebling, Josimeri
DOI：
10.1002/jbm.b.30320
发表时间：
2005-08-01
影响因子：
3.4
作者：
Bae, JH;Cho, BH;Kim, OY
通讯作者：
Kim, OY
影响因子：
14
作者：
Krifka, Stephanie;Petzel, Christine;Schweikl, Helmut
通讯作者：
Schweikl, Helmut