The Bacillus subtilis DnaD and DnaB proteins exhibit different DNA remodelling activities.
The Bacillus subtilis DnaD and DnaB proteins exhibit different DNA remodelling activities.
复制标题
DOI：
10.1016/j.jmb.2005.05.065
复制
发表时间：
2005-08-05
影响因子：
5.6
通讯作者：
Soultanas, P
中科院分区：
文献类型：
--
作者：
Zhang, WK;Carneiro, MJVM;Turner, IJ;Allen, S;Roberts, CJ;Soultanas, P
引物体蛋白级联将复制解旋酶加载到DNA上。在枯草芽孢杆菌中，一个假定的涉及DnaD-DnaB-DnaI蛋白质的灵长类级联反应被认为参与了DnaA和PriA依赖性的复制解旋酶DnaC加载到DNA上。最近，我们发现DnaD具有全局重塑DNA活性，这表明在细菌类核结构中具有更广泛的作用。在这里，我们表明，DnaB形成了一个“正方形”的四聚体，在中心有一个洞，并建议其与DNA的相互作用的模型。它具有与DnaD不同的整体DNA重塑活性。而DnaD打开超螺旋DNA，DnaB作为横向压缩蛋白。这两种竞争活动可以共同作用于超螺旋质粒，形成两个拓扑结构不同的极点;一个用DnaB压缩，另一个用DnaD打开。我们建议，DnaB和DnaD的主要作用是在细菌类核结构的控制和调制，其对DNA复制的启动的影响是一个次要的作用，从染色体DNA的结构扰动。
Primosomal protein cascades load the replicative helicase onto DNA. In Bacillus subtilis a putative primosomal cascade involving the DnaD-DnaB-DnaI proteins has been suggested to participate in both the DnaA and PriA-dependent loading of the replicative helicase DnaC onto the DNA. Recently we discovered that DnaD has a global remodelling DNA activity suggesting a more widespread role in bacterial nucleoid architecture. Here, we show that DnaB forms a “square-like” tetramer with a hole in the centre and suggest a model for its interaction with DNA. It has a global DNA remodelling activity that is different from that of DnaD. Whereas DnaD opens up supercoiled DNA, DnaB acts as a lateral compaction protein. The two competing activities can act together on a supercoiled plasmid forming two topologically distinct poles; one compacted with DnaB and the other open with DnaD. We propose that the primary roles of DnaB and DnaD are in bacterial nucleoid architecture control and modulation, and their effects on the initiation of DNA replication are a secondary role resulting from architectural perturbations of chromosomal DNA.
登录
查看更多内容
影响因子：
3.6
作者：
Schneider, R;Travers, A;Muskhelishvili, G
通讯作者：
Muskhelishvili, G
影响因子：
3.5
作者：
Turner, IJ;Scott, DJ;Allen, S;Roberts, CJ;Soultanas, P
通讯作者：
Soultanas, P
影响因子：
14.9
作者：
Dame, RT;Wyman, C;Goosen, N
通讯作者：
Goosen, N
影响因子：
3.6
作者：
Bruand, C;Velten, M;Polard, P
通讯作者：
Polard, P
DOI：
10.1073/pnas.84.3.653
发表时间：
1987-02-01
影响因子：
11.1
作者：
HOSHINO, T;MCKENZIE, T;SUEOKA, N
通讯作者：
SUEOKA, N