The Awesome Power of Yeast Evolutionary Genetics: New Genome Sequences and Strain Resources for the Saccharomyces sensu stricto Genus.
The Awesome Power of Yeast Evolutionary Genetics: New Genome Sequences and Strain Resources for the Saccharomyces sensu stricto Genus.
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酵母进化遗传学的令人敬畏的力量：Saccharomyces sensu stricto属的新基因组序列和应变资源。
DOI：
10.1534/g3.111.000273
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发表时间：
2011-06
期刊：
影响因子：
--
通讯作者：
Hittinger CT
中科院分区：
文献类型：
--
作者：
Scannell DR;Zill OA;Rokas A;Payen C;Dunham MJ;Eisen MB;Rine J;Johnston M;Hittinger CT
高质量、注释良好的基因组序列和标准化的实验室菌株为实验和进化研究提供了动力。我们提出了改进的基因组序列的三种严格意义上的酵母菌：S。巴亚努斯湾uvarum（CBS 7001）、S. kudriavzevii（IFO 1802T和ZP 591）和S.（IFO 1815T）的基因组，并将其与S.酿酒酵母和酿酒酵母。矛盾的。通过将数百万个短DNA序列读数与先前发表的桑格鸟枪读数组装在一起而获得的新序列，比先前可用的基因组序列具有更大的长程连续性和更少的缺口。新的基因预测在五种最常研究的酵母菌中定义了一组5261个蛋白质编码的直系同源物，从而能够重新检查酵母基因进化的速度和模式，并改进对物种特异性增益和损失的推断。为了便于实验研究，我们产生了遗传标记，稳定的单倍体菌株，所有这三个酵母属物种。这些几乎完整的基因组序列和基因标记菌株的集合为基因功能、代谢和进化的比较研究提供了有价值的工具集，并使严格意义上的酵母成为实验上最易处理的模型属。这些资源可通过www.sophomycesSensuiteto.org免费获取。
High-quality, well-annotated genome sequences and standardized laboratory strains fuel experimental and evolutionary research. We present improved genome sequences of three species of Saccharomyces sensu stricto yeasts: S. bayanus var. uvarum (CBS 7001), S. kudriavzevii (IFO 1802T and ZP 591), and S. mikatae (IFO 1815T), and describe their comparison to the genomes of S. cerevisiae and S. paradoxus. The new sequences, derived by assembling millions of short DNA sequence reads together with previously published Sanger shotgun reads, have vastly greater long-range continuity and far fewer gaps than the previously available genome sequences. New gene predictions defined a set of 5261 protein-coding orthologs across the five most commonly studied Saccharomyces yeasts, enabling a re-examination of the tempo and mode of yeast gene evolution and improved inferences of species-specific gains and losses. To facilitate experimental investigations, we generated genetically marked, stable haploid strains for all three of these Saccharomyces species. These nearly complete genome sequences and the collection of genetically marked strains provide a valuable toolset for comparative studies of gene function, metabolism, and evolution, and render Saccharomyces sensu stricto the most experimentally tractable model genus. These resources are freely available and accessible through www.SaccharomycesSensuStricto.org.
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影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
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Chou JY;Hung YS;Lin KH;Lee HY;Leu JY
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Dujon, B;Sherman, D;Souciet, JL
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影响因子：
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Byrne KP;Wolfe KH
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