A new class of protein sensor links spirochete pleomorphism, persistence, and chemotaxis.
A new class of protein sensor links spirochete pleomorphism, persistence, and chemotaxis.
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致病性螺旋体可以改变其形态和行为以感染宿主并在宿主体内存活。以往的研究表明，螺旋体的致病机制与其“圆体”和生物膜的形成以及趋化性有关。在这里，我们报告了这些细胞状态之间的直接联系，涉及一类新的蛋白质传感器，迄今为止功能尚不清楚。使用冷冻电子显微镜，遗传学，行为分析和分子建模，我们证明了螺旋体调节这些行为的小分子S-腺苷甲硫氨酸（SAM）通过SAM传感器，锚定到趋化性阵列。此外，我们建立了一个改进的模型，圆体形成，现在包括在日志阶段的增长特征。一类新的细菌蛋白传感器监测细胞内S-腺苷甲硫氨酸水平，以调节细胞形态，趋化性和生物膜形成。这些行为的同时调节使细菌病原体能够在其生态位内生存。该传感器，例如齿垢密螺旋体CheWS，锚定在趋化性阵列上，其传感器结构域位于趋化性环下方。该位置可以允许传感器直接与趋化性组氨酸激酶CheA相互作用。总的来说，这些数据建立了一个关键的作用，咀嚼在发病机制，并进一步说明了研究非典型的趋化蛋白的影响。
Pathogenic spirochetes can alter their morphologies and behaviors to infect and survive within their hosts. Previous reports demonstrate that the formation of the so-called “round bodies” and biofilms, and chemotaxis are involved in spirochete pathogenesis. Here, we report a direct link between these cellular states that involve a new class of protein sensor with hitherto unclear function. Using cryo-electron microscopy, genetics, behavioral assays, and molecular modeling, we demonstrate that spirochetes regulate these behaviors in response to the small molecule S-adenosylmethionine (SAM) via a SAM sensor that is anchored to chemotaxis arrays. Furthermore, we establish an improved model for round body formation that now includes characterizations during log phase growth. A new class of bacterial protein sensors monitors intracellular levels of S-adenosylmethionine to modulate cell morphology, chemotaxis, and biofilm formation. Simultaneous regulation of these behaviors enables bacterial pathogens to survive within their niche. This sensor, exemplified by Treponema denticola CheWS, is anchored to the chemotaxis array and its sensor domain is located below the chemotaxis rings. This position may allow the sensor to directly interact with the chemotaxis histidine kinase CheA. Collectively, these data establish a critical role of CheWS in pathogenesis and further illustrate the impact of studying non-canonical chemotaxis proteins.
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影响因子：
4.3
作者：
Ortega DR;Mo G;Lee K;Zhou H;Baudry J;Dahlquist FW;Zhulin IB
通讯作者：
Zhulin IB
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
6.4
作者：
Huang, Zhou;Wang, Yun-Hao;Liu, Shuang-Jiang
通讯作者：
Liu, Shuang-Jiang
影响因子：
4.8
作者：
Garcia-Fontana, Cristina;Antonio Reyes-Darias, Jose;Krell, Tino
通讯作者：
Krell, Tino
影响因子：
2.8
作者：
Alban, PS;Johnson, PW;Nelson, DR
通讯作者：
Nelson, DR