Dorsal root ganglion stimulation of injured sensory neurons in rats rapidly eliminates their spontaneous activity and relieves spontaneous pain.
Dorsal root ganglion stimulation of injured sensory neurons in rats rapidly eliminates their spontaneous activity and relieves spontaneous pain.
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DOI：
10.1097/j.pain.0000000000002284
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发表时间：
2021-12-01
期刊：
影响因子：
7.4
通讯作者：
Pan B
中科院分区：
文献类型：
--
作者：
Chao D;Mecca CM;Yu G;Segel I;Gold MS;Hogan QH;Pan B
背根神经节场刺激（GFS）通过使用依赖性阻断通过感觉神经元T接头的脉冲串来缓解诱发性和自发性神经病理性疼痛，对于C型单位，这在不到1分钟内完成，也部分阻断Ad单位。我们在脊神经结扎（SNL）大鼠模型中使用该工具选择性地阻断第五腰椎（L5）水平的轴突切断神经元的感觉神经元自发活动（SA），而不是阻断L4水平的未受伤但暴露于炎症的单位。在体内背根单单位记录SNL后显示增加SA在L5单位，但不是L4单位。神经节场刺激阻断了这种SA。在L5背根神经节传递的神经节场刺激阻断了机械性痛觉过敏行为、机械性异常性疼痛和由条件性位置偏爱指示的持续自发性疼痛，而在L4的GFS阻断了诱发性疼痛行为，但不阻断自发性疼痛。在体脊髓背角（DH）宽动态范围神经元的单单位记录显示SNL后SA升高，在L5水平上被GFS降低，但在L4水平上没有被GFS降低。此外，在皮肤机械刺激过程中，L5 GFS而不是L4 GFS增加DH单位的机械阈值，而L5 GFS在降低诱发放电率方面超过L4 GFS。我们的研究结果表明，SA在受伤的神经元支持增加放电DH宽动态范围神经元，有助于痛觉过敏，异常性疼痛，持续疼痛。神经节场刺激神经损伤后的镇痛作用至少部分归因于阻断这种SA的传播。
Dorsal root ganglion field stimulation (GFS) relieves evoked and spontaneous neuropathic pain by use-dependent blockade of impulse trains through the sensory neuron T-junction, which becomes complete within less than 1 minute for C-type units, also with partial blockade of Ad units. We used this tool in the spinal nerve ligation (SNL) rat model to selectively block sensory neuron spontaneous activity (SA) of axotomized neurons at the fifth lumbar (L5) level vs blockade of units at the L4 level that remain uninjured but exposed to inflammation. In vivo dorsal root single-unit recordings after SNL showed increased SA in L5 units but not L4 units. Ganglion field stimulation blocked this SA. Ganglion field stimulation delivered at the L5 dorsal root ganglion blocked mechanical hyperalgesia behavior, mechanical allodynia, and ongoing spontaneous pain indicated by conditioned place preference, whereas GFS at L4 blocked evoked pain behavior but not spontaneous pain. In vivo single-unit recordings of spinal cord dorsal horn (DH) wide-dynamic-range neurons showed elevated SA after SNL, which was reduced by GFS at the L5 level but not by GFS at the L4 level. In addition, L5 GFS, but not L4 GFS, increased mechanical threshold of DH units during cutaneous mechanical stimulation, while L5 GFS exceeded L4 GFS in reducing evoked firing rates. Our results indicate that SA in injured neurons supports increased firing of DH wide-dynamic-range neurons, contributing to hyperalgesia, allodynia, and ongoing pain. Ganglion field stimulation analgesic effects after nerve injury are at least partly attributable to blocking propagation of this SA.
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影响因子：
16.2
作者：
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通讯作者：
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影响因子：
7.4
作者：
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