Salinity-induced Physiological Modification in the Callus from Halophyte Nitraria tangutorum Bobr.
Salinity-induced Physiological Modification in the Callus from Halophyte Nitraria tangutorum Bobr.
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盐度诱导的盐生白刺愈伤组织的生理改变。
DOI：
10.1007/s00344-010-9158-8
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发表时间：
2010-06
影响因子：
4.8
通讯作者：
An, Lizhe
中科院分区：
文献类型：
--
作者：
Yang, Yingli;Wei, Xueling;Shi, Ruxia;Fan, Qing;An, Lizhe
荒漠盐生植物唐古特白刺（Nitraria tangutorum Bobr.）的生理适应机制目前尚不清楚。对环境以唐古特白刺（Nitraria tangutorum Bobr.）研究了盐胁迫下的生理反应及一氧化氮（NO）对过氧化氢酶（CAT）活性的调节作用。在低盐（50和100 mM NaCl）条件下，愈伤组织的干重和可溶性蛋白质含量增加，而在200 mM NaCl条件下，这两个生长参数显著降低，脯氨酸和可溶性碳水化合物含量也显著增加。此外，短期应力从50 mM NaCl和应用较低的硝普钠（SNP，NO供体）浓度导致丙二醛（MDA）的水平降低。相反，较高浓度的NaCl和SNP诱导白刺显著的氧化损伤。愈伤组织基于荧光探针DAF-FM DA的分析表明，NaCl和SNP处理导致愈伤组织细胞中NO水平的提高。此外，NO清除剂2-（4-羧基苯基）-4，4，5，5-四甲基咪唑啉-1-氧基-3-氧化物（c-PTIO）可降低内源NO浓度，并消除50 mM-NaCl处理下干重的增加和MDA水平的降低。盐胁迫下CAT活性升高，c-PTIO或一氧化氮合酶抑制剂Nω-nitro-l-arginine处理可缓解50 mM-NaCl对CAT活性的影响。建议唐古特白刺（Nitraria tangutorum Bobr.）愈伤组织表现出对低盐胁迫的耐受性。我们还表明，增加NO生成响应盐度可能与调节生长，保护免受氧化损伤，并在白刺CAT活性的激发。盐胁迫下的愈伤组织。
Little is known about the physiological adaptation mechanisms of the desert halophyte Nitraria tangutorum Bobr. to the environment. In this study, callus from Nitraria tangutorum Bobr. was used to investigate physiological responses to salinity and the regulatory function of nitric oxide (NO) on catalase (CAT) activity. Increased dry weight and soluble proteins were observed in the callus exposed to lower salinity (50 and 100 mM NaCl), whereas 200 mM NaCl led to significant decreases of these two growth parameters, and the levels of proline and soluble carbohydrates also were enhanced under NaCl treatment. In addition, short-term stress from 50 mM NaCl and the application of lower sodium nitroprusside (SNP, a NO donor) concentration resulted in decreased levels of malondialdehyde (MDA). In contrast, higher concentrations of NaCl and SNP induced significant oxidative damage in Nitraria tangutorum Bobr. callus. Analysis based on the fluorescent probe DAF-FM DA revealed that NaCl and SNP treatment led to enhanced levels of NO in the callus cells. Moreover, the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) reduced endogenous NO concentrations and abolished the enhancement in dry weight and the decrease in MDA level under 50-mM-NaCl treatment. CAT activity increased under salt stress, and the 50-mM-NaCl effect was alleviated by treatment with c-PTIO or the nitric oxide synthase inhibitor Nω-nitro-l-arginine. We suggest that Nitraria tangutorum Bobr. callus exhibited tolerance to lower-salinity stress. We also showed that increased NO generation in response to salinity might be associated with regulation of growth, protection against oxidative damage, and excitation of CAT activity in Nitraria tangutorum Bobr. callus under salt stress.
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影响因子：
2.3
作者：
Kerepesi, I;Galiba, G
通讯作者：
Galiba, G
影响因子：
7.4
作者：
Beligni, MV;Fath, A;Jones, RL
通讯作者：
Jones, RL
DOI：
--
发表时间：
1995-02
期刊：
Journal of Genetics and Breeding
影响因子：
--
作者：
A. Hakimi;P. Monneveux;G. Galiba
通讯作者：
A. Hakimi;P. Monneveux;G. Galiba
红芸豆在盐胁迫下，葡萄糖-6-磷酸脱氢酶在一氧化氮相关的氧化应激防御中发挥着关键作用
影响因子：
4.9
作者：
Liu, Yinggao;Wu, Ruru;Bi, Yurong
通讯作者：
Bi, Yurong
影响因子：
3.5
作者：
Clark, D;Durner, J;Klessig, DF
通讯作者：
Klessig, DF