A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice.
A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice.
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水稻花粉发育和植物生长都需要质体定位的五肽重复蛋白
DOI：
10.1038/s41598-017-10727-x
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发表时间：
2017-09-13
影响因子：
4.6
通讯作者：
Tu J
中科院分区：
文献类型：
--
作者：
Liu YJ;Liu X;Chen H;Zheng P;Wang W;Wang L;Zhang J;Tu J
一些与花粉发育有关的五肽重复序列（PPR）蛋白被报道为育性恢复（Rf）蛋白。然而，质体定位的PPR蛋白在植物雄性生殖中的作用还不清楚。在这里，我们描述了质体定位的PPR-SMR蛋白，OsPPR 676，这是植物生长和花粉发育所需的水稻。本研究通过双分子荧光互补分析证实OsPPR 676与新生多肽相关复合物（β-NAC）的β-亚基Osj 10 gBTF 3存在相互作用，表明Osj 10 gBTF 3和OsPPR 676可能参与了花粉发育的同一调控途径。与其它富含叶绿体的组织相比，OsPPR 676在花药中表达较弱，但在花粉发育的梅期和YM期，其在绒毡层中的表达相对较强。OsPPR 676的破坏导致植物生长迟缓和花粉部分不育。对不同突变株的表型分析表明，SMR结构域不是OsPPR 676功能所必需的。我们进一步证明了OsPPR 676是质体pB亚基产生所必需的，进而通过影响ATP合酶的活性在脂肪酸、碳水化合物和其他有机物质的生物合成中起关键作用。
Several mitochondrial-targeted pentatricopeptide repeat (PPR) proteins involved in pollen development have been reported to be fertility restorer (Rf) proteins. However, the roles of plastid-localized PPR proteins in plant male reproduction are poorly defined. Here, we described a plastid-localized PPR-SMR protein, OsPPR676, which is required for plant growth and pollen development in rice. In this study, OsPPR676 was confirmed to be an interacted protein with Osj10gBTF3, β-subunit of nascent polypeptide-associated complex (β-NAC), by bimolecular fluorescence complementation assays, indicating that both proteins are probably involved in the same regulatory pathway of pollen development. Compared with other chloroplast-rich tissues, OsPPR676 was only weakly expressed in anther, but in the Mei and YM stages of pollen development, its expression was relatively strong in the tapetum. Disruption of OsPPR676 resulted in growth retardation of plants and partial sterility of pollens. Phenotypic analysis of differentosppr676mutant lines implied that the SMR domain was not essential for the function of OsPPR676. We further demonstrated that OsPPR676 is essential for production of plastidatpBsubunit, and then plays crucial roles in biosynthesis of fatty acids, carbohydrates, and other organic matters via affecting activity of ATP synthase.
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DOI：
10.4061/2011/691735
发表时间：
2011
期刊：
Molecular biology international
影响因子：
--
作者：
Fukui K;Kuramitsu S
通讯作者：
Kuramitsu S
影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
Ronald PC
组蛋白单泛素化 1 和组蛋白单泛素化 2 介导的组蛋白 H2B 单泛素化通过调节水稻绒毡层降解相关基因参与花药发育
影响因子：
7.4
作者：
Cao, Hong;Li, Xiaoying;Liu, Yong-Xiu
通讯作者：
Liu, Yong-Xiu
影响因子：
3
作者：
Gui, Wen-Jun;Qu, Qian-Hui;Jiang, Tao
通讯作者：
Jiang, Tao
影响因子：
11.6
作者：
Jung, Ki-Hong;Han, Min-Jung;An, Gynheung
通讯作者：
An, Gynheung