Nonstructural protein 1 widespread RNA decay phenotype varies among coronaviruses.
Nonstructural protein 1 widespread RNA decay phenotype varies among coronaviruses.
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DOI：
10.1016/j.isci.2022.105887
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发表时间：
2023-01-20
期刊：
影响因子：
5.8
通讯作者：
Muller, Mandy
中科院分区：
文献类型：
--
作者：
Bermudez, Yahaira;Miles, Jacob;Muller, Mandy
冠状病毒非结构蛋白1(NSP1)对宿主基因表达的广泛重塑是冠状病毒-宿主接管的一个有充分证据和保守的方面。利用比较转录组学的方法，我们研究了不同NSP1蛋白之间转录靶点的多样性。此外，还进行了亲和纯化，然后进行了质谱分析，以确定不同NSP1蛋白之间的共同相互作用。尽管我们检测到了广泛的RNA不稳定，但密切相关的nsp1在靶基因的聚集方面几乎没有相似之处。我们观察到α-CoV229E和MERS NSP1在转录靶向方面有部分重叠，这可能表明了一种共同的靶向机制，因为MERS NSP1优先靶向核转录产物。我们的相互作用组数据显示NSP1相互作用之间有很大的变异性，229E NSP1，这里测试的最小的NSP1，与最多数量的宿主蛋白相互作用。虽然NSP1是一种非常保守的蛋白质，在不同的冠状病毒中具有保守的功能，但我们的数据表明，它对宿主细胞的确切影响是病毒特有的。多种冠状病毒的NSP1改变宿主mRNA的表达不同冠状病毒的NSP1之间的转录靶向是高度不同的宿主-NSP1蛋白的相互作用在不同的冠状病毒中是独特的微生物学；分子微生物学；病毒学
Extensive remodeling of host gene expression by nonstructural protein 1 (nsp1) of coronaviruses is a well-documented and conserved aspect of coronavirus-host takeover. Using comparative transcriptomics we investigated the diversity of transcriptional targets between various nsp1 proteins. Additionally, affinity purification followed by mass spectrometry was implemented to identify common interactors between the different nsp1 proteins. Although we detected widespread RNA destabilization, closely related nsp1 showed little similarities in clustering of targeted genes. We observed a partial overlap in transcriptional targeting between α-CoV 229E and MERS nsp1, which may suggest a common targeting mechanism, as MERS nsp1 preferentially targets nuclear transcripts. Our interactome data show great variability between nsp1 interactions, with 229E nsp1, the smallest nsp1 tested here, interacting with the most number of host proteins. Although nsp1 is a rather well-conserved protein with conserved functions across different coronaviruses, our data indicate that its precise effects on the host cell are virus specific. nsp1s from multiple coronaviruses alter expression of host mRNA Transcriptional targeting between nsp1 of different coronaviruses is highly varied Host-nsp1 protein interactions are unique among different coronaviruses Microbiology; Molecular microbiology; Virology
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