Electron paramagnetic resonance investigation on modulatory effect of benidipine on membrane fluidity of erythrocytes in essential hypertension
Electron paramagnetic resonance investigation on modulatory effect of benidipine on membrane fluidity of erythrocytes in essential hypertension
复制标题
电子顺磁共振研究贝尼地平对原发性高血压红细胞膜流动性的调节作用
DOI：
10.1007/s00380-007-1017-6
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发表时间：
2008
影响因子：
1.5
通讯作者：
K. Tsuda
中科院分区：
文献类型：
--
作者：
K. Tsuda
研究表明，贝尼地平是一种长效钙 (Ca) 通道阻滞剂，可通过增加一氧化氮 (NO) 的产生来发挥其对血管疾病的保护作用。本研究的目的是调查口服贝尼地平是否可能影响原发性高血压患者的膜功能。我们使用电子顺磁共振（EPR）和自旋标记方法测量了红细胞的膜流动性。在使用从健康志愿者获得的红细胞的初步研究中，贝尼地平降低了体外 EPR 光谱中 5-硝基氧硬脂酸酯 (5-NS) 的有序参数 (S) 和 16-NS 的峰高比 (ho/h-1)。研究结果表明，贝尼地平增加了膜流动性并改善了红细胞的微粘度。此外，还证明贝尼地平对红细胞膜流动性的影响被 NO 底物 L-精氨酸显着增强。在单独的系列研究中，我们观察到口服贝尼地平 4 周显着增加了高血压受试者的红细胞膜流动性，同时血浆 NO 代谢物水平增加。本研究结果表明，贝尼地平可能在一定程度上通过NO依赖性机制增加体外和体内红细胞膜的流动性并改善红细胞的微粘度。此外，强烈建议口服贝尼地平可能对高血压受试者的红细胞流变行为和微循环改善产生有益影响。
It has been shown that benidipine, a long-lasting calcium (Ca) channel blocker, may exert its protective effect against vascular disorders by increasing nitric oxide (NO) production. The purpose of the present study was to investigate whether orally administered benidipine might influence the membrane function in patients with essential hypertension. We measured the membrane fluidity of erythrocytes by using an electron paramagnetic resonance (EPR) and spin-labeling method. In the preliminary study using erythrocytes obtained from healthy volunteers, benidipine decreased the order parameter (S) for 5-nitroxide stearate (5-NS) and the peak height ratio (ho/h-1) for 16-NS in the EPR spectra in vitro. The finding indicated that benidipine increased the membrane fluidity and improved the microviscosity of erythrocytes. In addition, it was demonstrated that the effect of benidipine on membrane fluidity of erythrocytes was significantly potentiated by the NO-substrate, L-arginine. In the separate series of the study, we observed that orally administered benidipine for 4 weeks significantly increased the membrane fluidity of erythrocytes with a concomitant increase in plasma NO metabolite levels in hypertensive subjects. The results of the present study demonstrated that benidipine might increase the membrane fluidity and improve the microviscosity of erythrocytes both in vitro and in vivo, to some extent, by the NO-dependent mechanism. Furthermore, it is strongly suggested that orally administered benidipine might have a beneficial effect on the rheologic behavior of erythrocytes and the improvement of the microcirculation in hypertensive subjects.
NO 代谢物在红细胞中的积累与二氧化碳和碳酸氢盐浓度成比例。
DOI：
10.1152/ajpheart.2000.279.2.h852
发表时间：
2000
期刊：
American journal of physiology. Heart and circulatory physiology
影响因子：
--
作者：
Recchia,FA;Vogel,TR;Hintze,TH
通讯作者：
Hintze,TH
DOI：
--
发表时间：
2000
期刊：
--
影响因子：
--
作者：
Zhi-wei Yang;A. Gebrewold;Maja Nowakowski;B. T. Altura;B. Altura
通讯作者：
Zhi-wei Yang;A. Gebrewold;Maja Nowakowski;B. T. Altura;B. Altura
影响因子：
4.5
作者：
Yao,JK;vanKammen,DP
通讯作者：
vanKammen,DP