Genome-wide identification of long non-coding RNA and mRNA profiling using RNA sequencing in subjects with sensitive skin.
Genome-wide identification of long non-coding RNA and mRNA profiling using RNA sequencing in subjects with sensitive skin.
复制标题
使用 RNA 测序对敏感皮肤受试者进行长非编码 RNA 和 mRNA 分析的全基因组鉴定
DOI：
10.18632/oncotarget.23147
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发表时间：
2017-12-29
期刊：
影响因子：
--
通讯作者：
He L
中科院分区：
文献类型：
--
作者：
Yang L;Lyu L;Wu W;Lei D;Tu Y;Xu D;Feng J;He L
敏感性皮肤（SS）是一种主观皮肤高反应性的病症。长链非编码RNA（lncRNA）在SS受试者中的作用尚不清楚。因此，本研究的目的是提供SS受试者中mRNA和lncRNA的全面概况。基因本体论（GO）和京都基因和基因组百科全书（KEGG）分析显示了相关蛋白编码基因的特征。此外，构建了lncRNA和mRNA的共表达网络，以确定潜在的潜在调控靶点;通过SS患者和正常样本的定量实时PCR（qRT-PCR）和RNA-seq分析验证了结果。与正常皮肤组相比，在SS组中共鉴定出266个新的lncRNA和6750个注释的lncRNA。结果表明，在71个lncRNA转录本和2615个mRNA转录本中，差异表达显著（P < 0.05）。SS样品的热特征可以与正常皮肤样品区分开，而存在于富集途径中的大多数基因是参与粘着斑、PI 3 K-Akt信号传导和癌症相关途径的基因。选择5个转录本进行qRT-PCR分析，结果与RNA-seq一致。结果提示LNC_000265可能在SS患者表皮屏障结构中发挥作用。这些数据表明，新的基因和途径，可能参与了SS的发病机制，并突出了潜在的目标，可用于个性化治疗的应用。
Sensitive skin (SS) is a condition of subjective cutaneous hyper-reactivity. The role of long non-coding RNAs (lncRNAs) in subjects with SS is unclear. Therefore, the aim of the present study was to provide a comprehensive profile of the mRNAs and lncRNAs in subjects with SS. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis presented the characteristics of associated protein-coding genes. In addition, a co-expression network of lncRNA and mRNA was constructed to identify potential underlying regulation targets; the results were verified by quantitative real-time PCR (qRT-PCR) and RNA-seq analyses in patients with SS and normal samples. Compared with the normal skin group, 266 novel lncRNAs and 6750 annotated lncRNAs were identified in the SS group. A total of 71 lncRNA transcripts and 2615 mRNA transcripts were differentially expressed (P < 0.05). The heat signature of the SS samples could be distinguished from the normal skin samples, whereas the majority of the genes that were present in enriched pathways were those that participated in focal adhesion, PI3K-Akt signaling, and cancer-related pathways. Five transcripts were selected for qRT-PCR analysis and the results were consistent with RNA-seq. The results suggested that LNC_000265 may play a role in the epidermal barrier structure of patient with SS. The data suggest novel genes and pathways that may be involved in the pathogenesis of SS and highlight potential targets that could be used for individualized treatment applications.
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影响因子：
11.1
作者：
Bonasio R;Shiekhattar R
通讯作者：
Shiekhattar R
影响因子：
7.8
作者：
Furuse, Mikio;Hata, Masaki;Furuse, Kyoko;Yoshida, Yoko;Haratake, Akinori;Sugitani, Yoshinobu;Noda, Tetsuo;Kubo, Akiharu;Tsukita, Shoichiro
通讯作者：
Tsukita, Shoichiro
DOI：
10.1159/000441541
发表时间：
2016-01-01
期刊：
SKIN BARRIER FUNCTION
影响因子：
--
作者：
Brandner, Johanna M.
通讯作者：
Brandner, Johanna M.
影响因子：
3.6
作者：
Buhe, Virginie;Vie, Katell;Misery, Laurent
通讯作者：
Misery, Laurent
影响因子：
10.5
作者：
Cabili, Moran N.;Trapnell, Cole;Rinn, John L.
通讯作者：
Rinn, John L.