CD55 is over-expressed in the tumour environment.
CD55 is over-expressed in the tumour environment.
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DOI：
10.1054/bjoc.2000.1570
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发表时间：
2001-01-05
影响因子：
8.8
通讯作者：
Durrant LG
中科院分区：
文献类型：
--
作者：
Li L;Spendlove I;Morgan J;Durrant LG
CD55 是一种保护细胞免受补体介导的攻击的蛋白质。 791Tgp72 是一种抗原，已成功用作肿瘤成像和癌症疫苗的靶标。 791Tgp72最近被鉴定为CD55。因此研究了肿瘤环境中CD55的定量表达。与正常细胞相比，肿瘤细胞的 CD55 细胞表面表达增加了 4-100 倍。结直肠肿瘤的免疫组织化学染色也显示基质中 CD55 高表达。为了检查基质 CD55 的来源，测量了上皮细胞和内皮细胞产生细胞外 CD55 的能力。肿瘤细胞系将 CD55 沉积到其细胞外基质 (ECM) 中，与其细胞表面表达成正比。相比之下，来自 HUVEC 细胞的 ECM 含有大量 CD55，尽管其细胞表面表达的 CD55 水平较低。此外，暴露于 VEGF 会增加 HUVEC 细胞上 CD55 的表达。尽管尚不清楚为什么 CD55 在肿瘤环境中上调，但其在肿瘤细胞和相关内皮细胞上的高表达水平可能解释了为什么它是成像和免疫治疗的良好靶点。 © 2001 癌症研究运动 http://www.bjcancer.com
CD55 is a protein that protects cells from complement-mediated attack. 791Tgp72 is an antigen which has been used succesfully as a target for both tumour imaging and cancer vaccines. 791Tgp72 has recently been identified as CD55. Quantitative expression of CD55 in the tumour environment was therefore studied. Tumour cells showed a 4–100-fold increase in CD55 cell surface expression when compared to normal cells. Immunohistochemical staining of colorectal tumours also revealed high expression of CD55 in the stroma. To examine the source of this stromal CD55 the ability of both epithelial cells and endothelial cells to produce extracellular CD55 was measured. Tumour cell lines deposit CD55 into their extracellular matrix (ECM) in direct proportion to their cell surface expression. In contrast the ECM from HUVEC cells contained large amounts of CD55 despite expressing low levels of CD55 on their cell surface. Furthermore expression of CD55 on HUVEC cells was increased by exposure to VEGF. Although it remains unclear why CD55 is upregulated in the tumour environment its high level of expression on tumour cells and associated endothelium may explain why it is a good target for both imaging and immunotherapy. © 2001 Cancer Research Campaign http://www.bjcancer.com
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影响因子：
6.4
作者：
DENTON, GWL;DURRANT, LG;ROBINS, RA
通讯作者：
ROBINS, RA
影响因子：
15.9
作者：
JAFFE, EA;NACHMAN, RL;MINICK, CR
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MINICK, CR
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32.4
作者：
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影响因子：
2.7
作者：
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通讯作者：
MCKENZIE, IFC
影响因子：
4.1
作者：
TATE, CG;UCHIKAWA, M;ANSTEE, DJ
通讯作者：
ANSTEE, DJ