Molecular Cloning and Transcriptional Analysis of the Putative AGAMOUS Homolog AcAG in Onion (Allium cepa)
Molecular Cloning and Transcriptional Analysis of the Putative AGAMOUS Homolog AcAG in Onion (Allium cepa)
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洋葱 (Allium cepa) 中假定的 AGAMOUS 同源物 AcAG 的分子克隆和转录分析
DOI：
10.1007/s11105-013-0607-y
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发表时间：
2013-06
影响因子：
2.1
通讯作者：
Yong-Qin Wang
中科院分区：
文献类型：
--
作者：
Hong-You Li;Rui Zhao;Chan Wang;Li-Ying Zhang;Hong Zhao;Yong-Qin Wang
已知MADS-box基因家族成员在调节被子植物的花发育中起关键作用。为了扩大我们对单子叶植物花发育的认识，并阐明洋葱（Allium cepa）花发育的分子机制，我们分离了一个假定的c功能花同源同源同源基因AcAG （GenBank ID: JX974431）。基因组DNA分析表明，AcAG是洋葱基因组中的一个单拷贝基因，在进化过程中似乎失去了一个保守的内含子。序列比较和系统发育分析表明，AcAG基因是拟南芥AGAMOUS的同源基因，与单子叶植物中c系AG同源基因密切相关。此外，预测的AcAG蛋白在可变C端具有典型的MIKC结构域和两个高度保守的序列基序（AG基序I和II），但没有发现通常存在于eudicot euAG谱系中的n端延伸。通过半定量PCR检测，AcAG转录仅在花中检测到，在根、茎和叶等营养组织中不存在。实时荧光定量PCR分析表明，AcAG在花的雄蕊和心皮中特异表达，且在两个内生殖轮中具有时空表达模式。这与在其他物种中观察到的ag样基因的表达模式一致。上述结果表明，AcAG是拟南芥AGAMOUS的洋葱同源基因，可能在调节洋葱生殖器官发育中发挥重要作用。
Members of the MADS-box gene family are known to play critical roles in regulating flower development in angiosperms. To expand our knowledge of flower development in monocots, and to elucidate the molecular mechanisms involved in floral development in onion (Allium cepa), we have isolated a putative C-function flower homeotic identity gene AcAG (GenBank ID: JX974431). Genomic DNA analysis showed that AcAG is a single-copy gene in the onion genome that appears to have lost a conserved intron during evolution. Sequence comparison and phylogenetic analysis revealed that AcAG gene is a homolog of Arabidopsis AGAMOUS, and is closely related to C-lineage AG homologs in monocot species. Moreover, the predicted AcAG protein possesses a typical MIKC domain and two highly conserved sequence motifs (AG motifs I and II) specific to the C proteins in the variable C-terminus, but the N-terminal extension that is usually present in the eudicot euAG lineage was not found. Transcription of AcAG, determined by semi-quantitative PCR, was detected only in flowers and was absent in vegetative tissues such as roots, stems, and leaves. Real-time PCR analysis showed that AcAG was expressed specifically in stamens and carpels of flowers, and had spatial and temporal expression patterns in the two inner reproductive whorls. This is consistent with the expression patterns observed for AG-like genes in other species. All these results indicate that AcAG is the onion ortholog of Arabidopsis AGAMOUS, and that it may play an important role in regulating development of the reproductive organs in onion.
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影响因子：
--
作者：
Yamaguchi T;Hirano HY
通讯作者：
Hirano HY
影响因子：
56.9
作者：
SCHWARZSOMMER, Z;HUIJSER, P;SOMMER, H
通讯作者：
SOMMER, H
DOI：
--
发表时间：
2009
期刊：
--
影响因子：
--
作者：
Wu Haitao;Ma Rong-li;Liu HongJiong;Jiao Yansheng
通讯作者：
Wu Haitao;Ma Rong-li;Liu HongJiong;Jiao Yansheng
影响因子：
1.5
作者：
A. Tsaftaris;K. Pasentsis;A. Polidoros
通讯作者：
A. Tsaftaris;K. Pasentsis;A. Polidoros
影响因子：
2.4
作者：
I. Song;Toru M. Nakamura;T. Fukuda;J. Yokoyama;Takuro Ito;H. Ichikawa;Y. Horikawa;T. Kameya;A. Kanno
通讯作者：
I. Song;Toru M. Nakamura;T. Fukuda;J. Yokoyama;Takuro Ito;H. Ichikawa;Y. Horikawa;T. Kameya;A. Kanno