Binding dynamics of targeted microbubbles in response to modulated acoustic radiation force.
Binding dynamics of targeted microbubbles in response to modulated acoustic radiation force.
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DOI：
10.1088/0031-9155/59/2/465
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发表时间：
2014-01-20
影响因子：
3.5
通讯作者：
Mauldin FW Jr
中科院分区：
文献类型：
--
作者：
Wang S;Hossack JA;Klibanov AL;Mauldin FW Jr
分子靶向微泡的检测在基于超声的分子成像和靶向基因或药物递送中发挥着基础作用。在本文中，提出了一个经验模型，描述了目标微泡响应于大血管中调制的声辐射力的结合动力学，并使用模拟组织流动模型进行了实验验证。较高的流速和微泡浓度导致特定结合的微泡更快的分离速率 (p < 0.001)。较高的时间平均声辐射力强度导致更快的附着速率和更高的特定结合微泡的饱和水平（p < 0.05）。辐射力停止后目标实验中残留微泡信号的水平是目标实验和对照实验之间唯一可靠地不同的响应参数（p < 0.05）。一个相关参数，残余微泡信号与饱和微泡信号的比率（Rresid），被提议作为独立于绝对声学信号幅度的测量，因此能够独立于控制测量可靠地检测目标粘附（p < 0.01）。这些发现表明，使用相对较短的成像方案（大约 3 分钟），可以实时增强对特定结合微泡的检测，而无需等待自由微泡的清除。
Detection of molecular targeted microbubbles plays a foundational role in ultrasound-based molecular imaging and targeted gene or drug delivery. In this paper, an empirical model describing the binding dynamics of targeted microbubbles in response to modulated acoustic radiation forces in large vessels is presented and experimentally verified using tissue-mimicking flow phantoms. Higher flow velocity and microbubble concentration led to faster detaching rates for specifically bound microbubbles (p < 0.001). Higher time-averaged acoustic radiation force intensity led to faster attaching rates and a higher saturation level of specifically bound microbubbles (p < 0.05). The level of residual microbubble signal in targeted experiments after cessation of radiation forces was the only response parameter that was reliably different between targeted and control experiments (p < 0.05). A related parameter, the ratio of residual-to-saturated microbubble signal (Rresid), is proposed as a measurement that is independent of absolute acoustic signal magnitude and therefore able to reliably detect targeted adhesion independently of control measurements (p < 0.01). These findings suggest the possibility of enhanced detection of specifically bound microbubbles in real-time, using relatively short imaging protocols (approximately 3 min), without waiting for free microbubble clearance.
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影响因子：
2.9
作者：
Gessner, Ryan C.;Streeter, Jason E.;Kothadia, Roshni;Feingold, Steven;Dayton, Paul A.
通讯作者：
Dayton, Paul A.
DOI：
10.1016/j.jconrel.2011.07.032
发表时间：
2011-12-20
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Caskey CF;Hu X;Ferrara KW
通讯作者：
Ferrara KW
影响因子：
3.4
作者：
Krasik, Ellen F.;Yee, Ka Lai;Hammer, Daniel A.
通讯作者：
Hammer, Daniel A.
DOI：
10.1109/tuffc.2010.1411
发表时间：
2010
期刊：
IEEE transactions on ultrasonics, ferroelectrics, and frequency control
影响因子：
--
作者：
Hu X;Zheng H;Kruse DE;Sutcliffe P;Stephens DN;Ferrara KW
通讯作者：
Ferrara KW
影响因子：
--
作者：
Klibanov, A. L.;Rychak, J. J.;Kaul, S.
通讯作者：
Kaul, S.