Antibacterial mechanism of ultrasound against Escherichia coli: Alterations in membrane microstructures and properties.
Antibacterial mechanism of ultrasound against Escherichia coli: Alterations in membrane microstructures and properties.
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DOI：
10.1016/j.ultsonch.2021.105509
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发表时间：
2021-05
影响因子：
8.4
通讯作者：
Guo M
中科院分区：
文献类型：
--
作者：
He Q;Liu D;Ashokkumar M;Ye X;Jin TZ;Guo M
US的抗菌作用呈时间和强度依赖性。US显著提高了大肠杆菌细胞对Teon的敏感性。超声明显增加外膜和内膜的通透性。超声可导致膜流动性降低和膜去极化。超声引起了一些膜组分的构象和组成的变化。这项研究旨在为细菌细胞膜对超声波照射的反应提供新的见解。将大肠杆菌O157：H7细胞置于不同的超声作用下(功率分别为、191、372和573W/cm2，频率为20 kHz，脉冲模式为2秒：2秒)，观察27分钟内细胞存活率的动态变化。随着超声强度的增加和作用时间的延长，大肠杆菌的菌落数下降了0.76-3.52logCFU/mL。通过百里香精油纳米乳剂(Teon)对超声处理的细胞对抗菌化合物敏感性的变化进行评估。结果表明，超声场对Teon的抗菌效果有一定的促进作用，使大肠杆菌数量减少2.16~7.10logcfu/mL。电子显微镜分析显示，经超声波处理的大肠杆菌细胞也表现出明显的形态和超微结构损伤，膜完整性被破坏，细胞结构畸形。用分光光度法检测细胞外膜和内膜通透性显著增加，胞浆渗漏和膜去极化。第一次，我们研究了在超声波作用下膜流动性的显著降低。此外，还利用红外光谱研究了超声场对某些细菌膜组分的影响。在这项研究中，多条证据有效地阐明了超声作用下细胞膜结构和性质的变化，这可以扩大我们对超声波抗菌分子机制的理解。
The antibacterial effect of US was in a time- and intensity-dependent manner. US remarkably increased the sensitivity of E. coli cells to TEON. US significantly increased the outer and inner membrane permeability. US resulted in membrane fluidity reduction and membrane depolarization. US caused conformational and compositional changes in some membrane components. This study was aimed at providing new insights on the response of bacterial cell membranes to ultrasound exposure. Escherichia coli (E. coli) O157:H7 cells were exposed to different ultrasound treatments (power intensities of 64, 191, 372, and 573 W/cm2, frequency of 20 kHz, pulsed mode of 2 sec: 2 sec) and the dynamic changes in cell viability within 27 min were assessed. With an increase in ultrasonic intensity and prolonged duration, a 0.76–3.52 log CFU/mL reduction in E. coli populations was attained. The alterations in the sensitivity of ultrasound-treated cells to antimicrobial compounds were evaluated by exposure to thyme essential oil nanoemulsion (TEON). The treatment reduced the E. coli population by 2.16–7.10 log CFU/mL, indicating the effects of ultrasonic field on facilitating the antibacterial efficacy of TEON. Ultrasonic-treated E. coli cells also displayed remarkable morphological and ultrastructural damages with destroyed membrane integrity and misshaped cell structures, which was observed by electron microscopy analysis. Significant increase in outer and inner membrane permeability, along with the cytoplasmic leakage and membrane depolarization were assessed utilizing spectrophotometry. For the first time, significant reduction in the membrane fluidity in response to ultrasound exposure were investigated. Additional efforts in exploring the effect of ultrasonic field on some bacterial membrane compositions were performed with infrared spectroscopy. In this study, multiple lines of evidence effectively served to elucidate the alterations on cellular membrane structure and property during exposure to sonication that could extend our understanding of the antimicrobial molecular mechanisms of ultrasound.
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影响因子：
5.2
作者：
Li J;Ma L;Liao X;Liu D;Lu X;Chen S;Ye X;Ding T
通讯作者：
Ding T
影响因子：
8.4
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
8.4
作者：
Jia, Yali;Yuan, Wenjuan;Wang, Xiaobing
通讯作者：
Wang, Xiaobing
影响因子：
3.9
作者：
Ji, Sang Hye;Ki, Se Hoon;Choi, Eun Ha
通讯作者：
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