Far from equilibrium dynamics of tracer particles embedded in a growing multicellular spheroid
Far from equilibrium dynamics of tracer particles embedded in a growing multicellular spheroid
复制标题
嵌入生长的多细胞球体中的示踪粒子远离平衡动力学
DOI：
10.1101/2020.03.28.013888
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
D. Thirumalai
中科院分区：
文献类型：
--
作者：
H. Samanta;S. Sinha;D. Thirumalai
癌细胞（CC）上的局部应力已通过将惰性示踪粒子（TP）嵌入生长中的多细胞球体中来测量。实验的效用要求TP不改变CC微环境。我们表明，使用理论和广泛的模拟，CC的增殖和凋亡，驱动远离平衡的TP的动态。在小于CC分裂时间的时间上，TP表现出亚扩散行为（均方位移，βTP < 1）。令人惊讶的是，在长时间限制下，由于持续定向运动的CC分裂次数，TP的运动是高扩散的（αTP > 2）。相比之下，CC增殖使它们的运动随机化，这是由于在短时间内的干扰导致的超扩散行为，在长时间内αCC超过1。令人惊讶的是，CC动力学和径向压力的TP的效果是可以忽略不计的，这表明TP是可靠的CC微环境的报告。
Local stresses on the cancer cells (CCs) have been measured by embedding inert tracer particles (TPs) in a growing multicellular spheroid. The utility of the experiments requires that the TPs do not alter the CC microenvironment. We show, using theory and extensive simulations, that proliferation and apoptosis of the CCs, drive the dynamics of the TPs far from equilibrium. On times less than the CC division times, the TPs exhibit sub-diffusive behavior (the mean square displacement, with βTP < 1). Surprisingly, in the long-time limit, the motion of the TPs is hyper-diffusive ( with αTP > 2) due to persistent directed motion for a number of CC division times. In contrast, CC proliferation randomizes their motion resulting from jamming at short times to super-diffusive behavior, with αCC exceeding unity, at long times. Surprisingly, the effect of the TPs on CC dynamics and radial pressure is negligible, suggesting that the TPs are reliable reporters of the CC microenvironment.
影响因子：
44.1
作者：
Marchetti, M. C.;Joanny, J. F.;Simha, R. Aditi
通讯作者：
Simha, R. Aditi
影响因子：
44.1
作者：
Bechinger, Clemens;Di Leonardo, Roberto;Volpe, Giovanni
通讯作者：
Volpe, Giovanni