Effects of age and platelet-rich plasma on ACL cell viability and collagen gene expression.
Effects of age and platelet-rich plasma on ACL cell viability and collagen gene expression.
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DOI：
10.1002/jor.21496
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发表时间：
2012-01
影响因子：
2.8
通讯作者：
Murray, Martha M.
中科院分区：
文献类型：
--
作者：
Cheng, Mingyu;Johnson, Victor M.;Murray, Martha M.
富血小板血浆（PRP）在大型动物模型中显示出在早期时间点刺激前交叉韧带（ACL）愈合的体内潜力。然而，在动物模型中，ACL的愈合潜力取决于动物年龄。在这项研究中，我们假设ACL细胞代谢、胶原基因表达和细胞对富血小板血浆（PRP）中生长因子的反应能力存在年龄依赖性差异。为了检验这一假设，ACL细胞从尿道未成熟的、青春期和成年猪获得，并在有或没有PRP的I型胶原水凝胶中培养14天。当在仅含胶原的水凝胶中培养时，来自成年猪的ACL细胞的凋亡率比未成熟猪低19%（p=0.001），细胞代谢活性比青春期猪高25%（p=0.006）。向胶原蛋白水凝胶中添加PRP导致来自未成熟和青春期动物的细胞中的细胞代谢活性显著增加、细胞凋亡率降低和胶原蛋白产生的刺激（对于所有比较，p<0.05），但对成年细胞的影响较小。这些结果表明，骨骼成熟可能会影响ACL细胞的代谢活性，凋亡，胶原蛋白的产生，并对PRP的反应。
Platelet-rich plasma (PRP) has shown in vivo potential to stimulate anterior cruciate ligament (ACL) healing at early time points in large animal models. However, in animal models, the healing potential of the ACL is dependent on animal age. In this study, we hypothesized that there are age-dependent differences in ACL cell metabolism, collagen gene expression and the ability of the cells to respond to growth factors in platelet-rich plasma (PRP). To test this hypothesis, ACL cells were obtained from skeletally immature, adolescent and adult pigs and cultured in a collagen type I hydrogel with or without PRP for 14 days. When cultured in collagen-only hydrogel, ACL cells from adult pigs had a 19% lower apoptotic rate as compared to immature pigs (p=0.001) and a 25% higher cellular metabolic activity as compared to adolescent pigs (p=0.006). The addition of PRP to the collagen hydrogel resulted in a significantly increased cellular metabolic activity, reduced apoptotic rate and stimulation of collagen production in the cells from the immature and adolescent animals (p<0.05 for all comparisons) but had less of an effect on adult cells. These findings suggest that skeletal maturity may influence ACL cells’ metabolic activity, apoptosis, collagen production, and response to PRP.
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影响因子：
1.9
作者：
Fufa, Duretti;Shealy, Blake;Murray, Martha M.
通讯作者：
Murray, Martha M.
影响因子：
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