Solid-phase extraction treatment is required for measurement of active glucagon-like peptide-1 by enzyme-linked immunosorbent assay kit affected by heterophilic antibodies.
Solid-phase extraction treatment is required for measurement of active glucagon-like peptide-1 by enzyme-linked immunosorbent assay kit affected by heterophilic antibodies.
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DOI：
10.1111/jdi.12896
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发表时间：
2019-03
影响因子：
3.2
通讯作者：
Inagaki N
中科院分区：
文献类型：
--
作者：
Hasegawa T;Komagata M;Hamasaki A;Harada N;Seino Y;Inagaki N
据报告，血液中的干扰物质可能影响人血浆中活性胰高血糖素样肽-1（GLP-1）的测定值。建议采用固相萃取（SPE）预处理来降低它们的影响，但这需要大量的成本和时间。然而，很少有调查的原因抑制物质和有关的方法，可以取代固相萃取。在本研究中，我们旨在寻找可能干扰活性GLP-1酶联免疫吸附试验（ELISA）的候选物质。使用两种使用不同抗体的活性GLP-1 ELISA试剂盒、多种提取载体和洗脱液对SPE方法进行评价。比较了有或无SPE以及有或无嗜异性阻断管的活性GLP-1浓度。与SPE预处理相比，未使用SPE时的活性GLP-1值通常更高。通过用异嗜性阻断管或不使用小鼠单克隆抗体的ELISA试剂盒进行预处理，消除了这种差异，并且不依赖于SPE。吸附到异嗜性阻断管载体上的物质可能会干扰活性GLP-1免疫测定。通过受嗜异性抗体影响的ELISA试剂盒测定活性GLP-1需要固相提取处理。
It is reported that interfering substances in the blood might influence the value for measurement of active glucagon‐like peptide‐1 (GLP‐1) in human plasma. Solid phase extraction (SPE) pretreatment is recommended to reduce their influence, but it requires a lot of cost and time. However, there is little investigation about causative inhibitory substances and about methods that can replace solid phase extraction. In the present study, we aimed to seek the candidate of the substances that might interfere with an active GLP‐1 enzyme‐linked immunosorbent assay (ELISA). Two kinds of active GLP‐1 ELISA kits using different antibodies, plural extraction carriers and elution solutions were used to evaluate the SPE method. Active GLP‐1 concentration was compared with or without SPE, and with or without a heterophilic blocking tube. Active GLP‐1 values were often higher without SPE compared with those with SPE pretreatment. This difference was eliminated by pretreatment with a heterophilic blocking tube or ELISA kits that did not use a mouse monoclonal antibody, and was independent of SPE. Substances absorbed to a heterophilic blocking tube carrier might interfere with an active GLP‐1 immunoassay. Solid‐phase extraction treatment is required for measurement of active GLP‐1 by an ELISA kit affected by heterophilic antibodies.
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影响因子：
2.9
作者：
Wewer Albrechtsen NJ;Bak MJ;Hartmann B;Christensen LW;Kuhre RE;Deacon CF;Holst JJ
通讯作者：
Holst JJ
影响因子：
3.2
作者：
Harada N;Hamasaki A;Yamane S;Muraoka A;Joo E;Fujita K;Inagaki N
通讯作者：
Inagaki N
影响因子：
8.2
作者：
NAUCK, M;STOCKMANN, F;CREUTZFELDT, W
通讯作者：
CREUTZFELDT, W
影响因子：
5.8
作者：
Dai, Haiqinq;Gustavson, Stephanie M.;Hirshberg, Boaz
通讯作者：
Hirshberg, Boaz
影响因子：
5.1
作者：
Gustavson, Stephanie M.;Dai, Haiqing;Calle, Roberto A.
通讯作者：
Calle, Roberto A.