Control of Maize Vegetative and Reproductive Development, Fertility, and rRNAs Silencing by HISTONE DEACETYLASE 108.
Control of Maize Vegetative and Reproductive Development, Fertility, and rRNAs Silencing by HISTONE DEACETYLASE 108.
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DOI：
10.1534/genetics.117.300625
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发表时间：
2018-04
期刊：
影响因子：
3.3
通讯作者：
Varotto S
中科院分区：
文献类型：
--
作者：
Forestan C;Farinati S;Rouster J;Lassagne H;Lauria M;Dal Ferro N;Varotto S
组蛋白脱乙酰酶(HDAC)催化去乙酰化组蛋白尾部的乙酰基，从而与DNA发生更紧密的相互作用，导致染色质状态难以转录。玉米HDA108属于Rpd3/HDA1 HDAC家族，在发育过程中普遍表达。新分离的hda108/hda108插入突变体表现出许多发育缺陷：株高显著降低，地上部和叶片发育发生变化，花序形态和育性发生变化。Western印迹分析和免疫定位实验表明，突变核中组蛋白乙酰化明显增加，而H3K9二甲基化显著减少。在hda108突变体中，CHG序列背景下的DNA甲基化状态和核糖体序列的转录水平也受到影响，而H3和H4乙酰化的丰富表现了重复和非重复转录上调的特点。对幼叶和花药的RNA-Seq分析表明，hda108突变体中转录因子的表达受到高度影响，花粉发育程序受到干扰。Hda108/hda108与表观调控突变体的杂交没有产生任何双突变后代，表明HDA108可能与不同的表观遗传途径存在遗传互作。我们的发现表明，HDA108直接参与了玉米发育、育性和基因组活性的表观遗传调控。
Histone deacetylases (HDACs) catalyze the removal of acetyl groups from acetylated histone tails that consequently interact more closely with DNA, leading to chromatin state refractory to transcription. Zea mays HDA108 belongs to the Rpd3/HDA1 HDAC family and is ubiquitously expressed during development. The newly isolated hda108/hda108 insertional mutant exhibited many developmental defects: significant reduction in plant height, alterations of shoot and leaf development, and alterations of inflorescence patterning and fertility. Western blot analyses and immunolocalization experiments revealed an evident increase in histone acetylation, accompanied by a marked reduction in H3K9 dimethylation, in mutant nuclei. The DNA methylation status, in the CHG sequence context, and the transcript level of ribosomal sequences were also affected in hda108 mutants, while enrichment in H3 and H4 acetylation characterizes both repetitive and nonrepetitive transcriptional up-regulated loci. RNA-Seq of both young leaf and anthers indicated that transcription factor expression is highly affected and that the pollen developmental program is disrupted in hda108 mutants. Crosses between hda108/hda108 and epiregulator mutants did not produce any double mutant progeny indicating possible genetic interactions of HDA108 with distinct epigenetic pathways. Our findings indicate that HDA108 is directly involved in regulation of maize development, fertility, and epigenetic regulation of genome activity.
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影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
8.8
作者：
Greer CB;Tanaka Y;Kim YJ;Xie P;Zhang MQ;Park IH;Kim TH
通讯作者：
Kim TH
影响因子：
7
作者：
Gent JI;Ellis NA;Guo L;Harkess AE;Yao Y;Zhang X;Dawe RK
通讯作者：
Dawe RK
影响因子：
7.2
作者：
Jang, IC;Pahk, YM;Kim, JK
通讯作者：
Kim, JK
影响因子：
56.9
作者：
Erhard, Karl F., Jr.;Stonaker, Jennifer L.;Hollick, Jay B.
通讯作者：
Hollick, Jay B.