Comparison of exoelectrogenic bacteria detected using two different methods: U-tube microbial fuel cell and plating method.
Comparison of exoelectrogenic bacteria detected using two different methods: U-tube microbial fuel cell and plating method.
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DOI：
10.1264/jsme2.me11205
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发表时间：
2012
影响因子：
2.2
通讯作者：
Lee T
中科院分区：
文献类型：
--
作者：
Yu J;Cho S;Kim S;Cho H;Lee T
在微生物燃料电池（MFC）中，将电子传递到电极的外生产电菌被认为是发电的关键因素。在这项研究中，U型管MFC和电镀方法用于从MFC的阳极分离外生产电菌。尽管使用了相同的来源，但根据分离方法鉴定了不同的微生物。变性凝胶梯度电泳（DGGE）分析表明，某些微生物在U型管MFC成为优势。优势菌与苍白杆菌属相似，属于α-变形菌门，在先前的研究中显示其能够作为外生产电菌发挥作用。三个分离物，一个属于芽孢杆菌属，两个属于类芽孢杆菌属，平板法鉴定为革兰氏阳性菌厚壁菌门（Firmicutes）。使用平板法将三种分离物接种到U型管MFC中，以分批模式操作并监测电流。从平板分离后接种每种分离物的所有U型管MFC产生的电流（峰值电流密度：3.6-16.3 mA/m2）低于混合培养的U型管MFC中的电流（48.3-62.6 mA/m2）。尽管分离株产生低电流，但发现各种细菌群参与电流产生。
In a microbial fuel cell (MFC), exoelectrogens, which transfer electrons to the electrode, have been regarded as a key factor for electricity generation. In this study, U-tube MFC and plating methods were used to isolate exoelectrogens from the anode of an MFC. Disparate microorganisms were identified depending on isolation methods, despite the use of an identical source. Denaturing gel gradient electrophoresis (DGGE) analysis showed that certain microorganisms became dominant in the U-tube MFC. The predominant bacterium was similar to Ochrobactrum sp., belonging to the Alphaproteobacteria, which was shown to be able to function as an exoelectrogen in a previous study. Three isolates, one affiliated with Bacillus sp. and two with Paenibacillus sp., were identified using the plating method, which belonged to the Gram-positive bacteria, the Firmicutes. The U-tube MFCs were inoculated with the three isolates using the plating method, operated in the batch mode and the current was monitored. All of the U-tube MFCs inoculated with each isolate after isolation from plates produced lower current (peak current density: 3.6–16.3 mA/m2) than those in U-tube MFCs with mixed culture (48.3–62.6 mA/m2). Although the isolates produced low currents, various bacterial groups were found to be involved in current production.
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影响因子：
3.8
作者：
Chung, Kyungmi;Okahe, Satoshi
通讯作者：
Okahe, Satoshi
影响因子：
2.1
作者：
Pham, CA;Jung, SJ;Chun, J
通讯作者：
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影响因子：
11.4
作者：
Xing, Defeng;Zuo, Yi;Logan, Bruce E.
通讯作者：
Logan, Bruce E.
影响因子：
5
作者：
Pham, The Hai;Boon, Nico;Rabaey, Korneel
通讯作者：
Rabaey, Korneel
影响因子：
11.4
作者：
Rabaey, K;Boon, N;Verstraete, W
通讯作者：
Verstraete, W