Mitochondrial proteome of mouse oocytes and cisplatin-induced shifts in protein profile.
Mitochondrial proteome of mouse oocytes and cisplatin-induced shifts in protein profile.
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小鼠卵母细胞线粒体蛋白质组和顺铂诱导的蛋白质谱变化
DOI：
10.1038/s41401-021-00687-4
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发表时间：
2021-12
影响因子：
8.2
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Zhang N;Sun AD;Sun SM;Yang R;Shi YY;Wang QL;Li XY;Ma JH;Yue W;Xie BT;Qiao J;Li M
线粒体是哺乳动物细胞的重要细胞器，为细胞提供能量并参与多种功能，如信号转导、细胞分化和细胞凋亡的调节。与体细胞中的线粒体相比，卵母细胞线粒体具有额外的重要性，因为它们是生殖细胞成熟所必需的，其中功能障碍可导致严重的遗传性疾病。因此，迫切需要一个系统的蛋白质组学谱的卵母细胞线粒体，以支持基础和临床研究，但这样的配置文件的收购已被阻碍的卵母细胞样品的稀有性和技术挑战与捕获线粒体蛋白从活卵母细胞。在这里，在这项工作中，使用邻近标记蛋白质组学，我们建立了一个特定的抗坏血酸过氧化物酶（APEX2）反应在活GV期小鼠卵母细胞，并确定了共158个蛋白质在卵母细胞线粒体。该蛋白质组包括内在的线粒体结构和功能的组件，参与与“细胞呼吸”，“ATP代谢”，“线粒体运输”等过程。此外，线粒体蛋白质组捕获后，卵母细胞暴露于抗肿瘤化疗顺铂揭示了差异变化的丰富的几个卵母细胞特异性线粒体蛋白。我们的研究提供了第一个描述的哺乳动物卵母细胞线粒体蛋白质组，我们知道，并进一步说明了与化疗药物相关的蛋白质丰度的动态变化。
Mitochondria are essential organelles that provide energy for mammalian cells and participate in multiple functions, such as signal transduction, cellular differentiation, and regulation of apoptosis. Compared with the mitochondria in somatic cells, oocyte mitochondria have an additional level of importance since they are required for germ cell maturation, dysfunction in which can lead to severe inherited disorders. Thus, a systematic proteomic profile of oocyte mitochondria is urgently needed to support the basic and clinical research, but the acquisition of such a profile has been hindered by the rarity of oocyte samples and technical challenges associated with capturing mitochondrial proteins from live oocytes. Here, in this work, using proximity labeling proteomics, we established a mitochondria-specific ascorbate peroxidase (APEX2) reaction in live GV-stage mouse oocytes and identified a total of 158 proteins in oocyte mitochondria. This proteome includes intrinsic mitochondrial structural and functional components involved in processes associated with “cellular respiration”, “ATP metabolism”, “mitochondrial transport”, etc. In addition, mitochondrial proteome capture after oocyte exposure to the antitumor chemotherapeutic cisplatin revealed differential changes in the abundance of several oocyte-specific mitochondrial proteins. Our study provides the first description of a mammalian oocyte mitochondrial proteome of which we are aware, and further illustrates the dynamic shifts in protein abundance associated with chemotherapeutic agents.
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影响因子：
14.8
作者：
Hung V;Udeshi ND;Lam SS;Loh KH;Cox KJ;Pedram K;Carr SA;Ting AY
通讯作者：
Ting AY
影响因子：
14.9
作者：
Ma J;Chen T;Wu S;Yang C;Bai M;Shu K;Li K;Zhang G;Jin Z;He F;Hermjakob H;Zhu Y
通讯作者：
Zhu Y
影响因子：
7.3
作者：
Alston, Charlotte L.;Rocha, Mariana C.;Lax, Nichola Z.;Turnbull, Doug M.;Taylor, Robert W.
通讯作者：
Taylor, Robert W.
影响因子：
6.7
作者：
Santos, TA;El Shourbagy, S;St John, JC
通讯作者：
St John, JC
影响因子：
8.4
作者：
Peng, B;English, MW;Newell, DR
通讯作者：
Newell, DR