New pathway of amine oxidation respiratory chain of Paracoccus denitrificans IFO 12442.

New pathway of amine oxidation respiratory chain of Paracoccus denitrificans IFO 12442.
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脱氮副球菌IFO 12442胺氧化呼吸链的新途径。

DOI:
10.1046/j.1432-1033.2001.01912.x
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发表时间:
2001
期刊:
European journal of biochemistry
影响因子:
--
通讯作者:
T. Ikeda
T. Ikeda
中科院分区:
--
文献类型:
--
作者:
K. Takagi;Keiko Yamamoto;K. Kano;T. Ikeda

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用生化和电化学方法鉴定了副球菌IFO 12442醌血红素蛋白胺脱氢酶(QH-AmDH)的生理电子受体。三种类型的血红素c-含蛋白质纯化与QH-AmDH从周质的正丁胺生长的细胞,只有组成型细胞色素c-550减少通过添加QH-AmDH和正丁胺。呼吸链的重建表明,细胞色素c-550介导的电子转移从QH-AmDH的末端氧化酶。这是嗜热假单胞菌胺氧化呼吸链的新途径。
The physiological electron acceptor of quinohemoprotein amine dehydrogenase (QH-AmDH) from Paracoccus denitrificans IFO 12442 was identified by biochemical and electrochemical methods. Of three types of heme c-containing proteins purified together with QH-AmDH from the periplasm of n-butylamine-grown cells, only constitutive cytochrome c-550 was reduced by the addition of QH-AmDH and n-butylamine. Reconstitution of the respiratory chain revealed that cytochrome c-550 mediates the electron transfer from QH-AmDH to the terminal oxidase. This is a new pathway of the amine oxidation respiratory chain of P. denitrificans.
DOI: 10.1016/0003-2697(87)90643-9
发表时间: 1987-02-15
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DOI: --
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