Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells.
Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells.
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DOI：
10.1186/1465-9921-6-135
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发表时间：
2005-11-11
影响因子：
5.8
通讯作者：
Peiris JS
中科院分区：
文献类型：
--
作者：
Chan MC;Cheung CY;Chui WH;Tsao SW;Nicholls JM;Chan YO;Chan RW;Long HT;Poon LL;Guan Y;Peiris JS
在香港，以及最近在越南、泰国和柬埔寨，都有与甲型流感H5 N1亚型相关的致命性人类呼吸道疾病的记录。我们以前证明，H5 N1病毒感染的患者血清中IP-10（干扰素-γ-诱导蛋白-10）水平异常高。此外，当与人类流感病毒亚型H1N1相比时，1997年（A/Hong Kong/483/97）（H5 N1/97）的H5 N1病毒在体外是来自原代人类巨噬细胞的促炎细胞因子（例如肿瘤坏死因子-α）和趋化因子（例如IP-10）的更有效的诱导剂，这表明细胞因子失调可能在H5 N1疾病的发病机制中起作用。由于呼吸道上皮细胞是流感病毒复制的主要靶细胞，因此研究H5 N1病毒在这些细胞中的细胞因子诱导谱是相关的。我们采用定量RT-PCR和ELISA方法比较了H5 N1病毒A/HK/483/97（H5 N1/97）、A/Vietnam/1194/04和A/Vietnam/3046/04（均为H5 N1/04）与人H1N1病毒在体外培养的人原代肺泡和支气管上皮细胞中诱导的细胞因子和趋化因子基因表达谱。我们证明，与人H1N1病毒相比，H5 N1/97和H5 N1/04病毒是体外原代人肺泡和支气管上皮细胞中IP-10、干扰素β、RANTES（受激活调节，正常T细胞表达和分泌）和白细胞介素6（IL-6）的更有效诱导剂。最近来自越南的H5 N1病毒（H5 N1/04）似乎比H5 N1/97病毒更能诱导IP-10。H5 N1/97和H5 N1/04亚型甲型流感病毒比H1N1亚型病毒更有效地诱导原代人呼吸道上皮细胞中的促炎细胞因子和趋化因子。我们认为这种细胞因子的过度诱导可能与人类H5 N1疾病的发病机制有关。
Fatal human respiratory disease associated with influenza A subtype H5N1 has been documented in Hong Kong, and more recently in Vietnam, Thailand and Cambodia. We previously demonstrated that patients with H5N1 disease had unusually high serum levels of IP-10 (interferon-gamma-inducible protein-10). Furthermore, when compared with human influenza virus subtype H1N1, the H5N1 viruses in 1997 (A/Hong Kong/483/97) (H5N1/97) were more potent inducers of pro-inflammatory cytokines (e.g. tumor necrosis factor-a) and chemokines (e.g. IP-10) from primary human macrophages in vitro, which suggests that cytokines dysregulation may play a role in pathogenesis of H5N1 disease. Since respiratory epithelial cells are the primary target cell for replication of influenza viruses, it is pertinent to investigate the cytokine induction profile of H5N1 viruses in these cells. We used quantitative RT-PCR and ELISA to compare the profile of cytokine and chemokine gene expression induced by H5N1 viruses A/HK/483/97 (H5N1/97), A/Vietnam/1194/04 and A/Vietnam/3046/04 (both H5N1/04) with that of human H1N1 virus in human primary alveolar and bronchial epithelial cells in vitro. We demonstrated that in comparison to human H1N1 viruses, H5N1/97 and H5N1/04 viruses were more potent inducers of IP-10, interferon beta, RANTES (regulated on activation, normal T cell expressed and secreted) and interleukin 6 (IL-6) in primary human alveolar and bronchial epithelial cells in vitro. Recent H5N1 viruses from Vietnam (H5N1/04) appeared to be even more potent at inducing IP-10 than H5N1/97 virus. The H5N1/97 and H5N1/04 subtype influenza A viruses are more potent inducers of proinflammatory cytokines and chemokines in primary human respiratory epithelial cells than subtype H1N1 virus. We suggest that this hyper-induction of cytokines may be relevant to the pathogenesis of human H5N1 disease.
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影响因子：
3.7
作者：
Seo, SH;Webby, R;Webster, RG
通讯作者：
Webster, RG
影响因子：
64.8
作者：
Kobasa, D;Takada, A;Kawaoka, Y
通讯作者：
Kawaoka, Y
影响因子：
168.9
作者：
Claas, ECJ;Osterhaus, ADME;Webster, RG
通讯作者：
Webster, RG
影响因子：
158.5
作者：
de Jong, MD;Van Cam, B;Farrar, J
通讯作者：
Farrar, J
DOI：
10.1016/s0140-6736(03)13413-7
发表时间：
2003-05-24
期刊：
Lancet (London, England)
影响因子：
--
作者：
Nicholls JM;Poon LL;Lee KC;Ng WF;Lai ST;Leung CY;Chu CM;Hui PK;Mak KL;Lim W;Yan KW;Chan KH;Tsang NC;Guan Y;Yuen KY;Peiris JS
通讯作者：
Peiris JS