The effect of age on the intestinal mucus thickness, microbiota composition and immunity in relation to sex in mice.
The effect of age on the intestinal mucus thickness, microbiota composition and immunity in relation to sex in mice.
复制标题
DOI：
10.1371/journal.pone.0184274
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Faas M
中科院分区：
文献类型：
--
作者：
Elderman M;Sovran B;Hugenholtz F;Graversen K;Huijskes M;Houtsma E;Belzer C;Boekschoten M;de Vos P;Dekker J;Wells J;Faas M
粘液层覆盖并保护肠道上皮细胞，使其不与微生物直接接触。这一粘液层不仅防止炎症，而且在微生物区系定植中起着至关重要的作用，表明粘液成分-微生物区系与肠道健康之间的复杂相互作用。然而，目前尚不清楚粘液层是否受年龄或性别的影响，以及这是否有助于报告的男性和女性肠道疾病的差异或随着年龄的增长。因此，在这项研究中，我们研究了年龄对粘液厚度、肠道微生物区系组成和免疫组成的影响与性别的关系。增龄引起的结肠粘液层的收缩与细菌的穿透和细菌与上皮的直接接触有关。此外，参与粘液生物合成的几个基因在老年小鼠中下调，特别是在雄性小鼠中，同时伴随着各种乳杆菌和未分类的IV和XIV梭状杆菌丰度的减少，以及潜在致病细菌普通拟杆菌的丰度增加。老年小鼠粘液和微生物区系的变化与免疫系统的激活增强有关，老年小鼠效应性T细胞的比例较高就说明了这一点。我们的数据有助于更好地了解粘液-微生物区系-与免疫反应之间的相互作用，并最终可能导致更有针对性的策略设计，以调节目标群体的粘液生产。
A mucus layer covers and protects the intestinal epithelial cells from direct contact with microbes. This mucus layer not only prevents inflammation but also plays an essential role in microbiota colonization, indicating the complex interplay between mucus composition-microbiota and intestinal health. However, it is unknown whether the mucus layer is influenced by age or sex and whether this contributes to reported differences in intestinal diseases in males and females or with ageing. Therefore, in this study we investigated the effect of age on mucus thickness, intestinal microbiota composition and immune composition in relation to sex. The ageing induced shrinkage of the colonic mucus layer was associated with bacterial penetration and direct contact of bacteria with the epithelium in both sexes. Additionally, several genes involved in the biosynthesis of mucus were downregulated in old mice, especially in males, and this was accompanied by a decrease in abundances of various Lactobacillus species and unclassified Clostridiales type IV and XIV and increase in abundance of the potential pathobiont Bacteroides vulgatus. The changes in mucus and microbiota in old mice were associated with enhanced activation of the immune system as illustrated by a higher percentage of effector T cells in old mice. Our data contribute to a better understanding of the interplay between mucus-microbiota-and immune responses and ultimately may lead to more tailored design of strategies to modulate mucus production in targeted groups.
登录
查看更多内容
影响因子：
14.2
作者：
Groschwitz, Katherine R.;Hogan, Simon P.
通讯作者：
Hogan, Simon P.
影响因子：
3.7
作者：
Gomez A;Luckey D;Yeoman CJ;Marietta EV;Berg Miller ME;Murray JA;White BA;Taneja V
通讯作者：
Taneja V
影响因子：
5.2
作者：
Geurts L;Lazarevic V;Derrien M;Everard A;Van Roye M;Knauf C;Valet P;Girard M;Muccioli GG;François P;de Vos WM;Schrenzel J;Delzenne NM;Cani PD
通讯作者：
Cani PD
影响因子：
29.4
作者：
Lelouard, Hugues;Fallet, Mathieu;Gorvel, Jean-Pierre
通讯作者：
Gorvel, Jean-Pierre
影响因子：
100.3
作者：
Cerf-Bensussan, Nadine;Gaboriau-Routhiau, Valerie
通讯作者：
Gaboriau-Routhiau, Valerie