Plant Growth Modulates Metabolites and Biological Activities in Retama raetam (Forssk.) Webb.
Plant Growth Modulates Metabolites and Biological Activities in Retama raetam (Forssk.) Webb.
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DOI：
10.3390/molecules23092177
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发表时间：
2018-08-29
期刊：
影响因子：
--
通讯作者：
Ksouri R
中科院分区：
文献类型：
--
作者：
Saada M;Falleh H;Catarino MD;Cardoso SM;Ksouri R
这项工作的重点是Retama raetam（Forssk）的变异。生物活性化合物作为植物周期的函数。主要结果表明，在营养生长阶段，它表现出最高的多不饱和脂肪酸百分比，沿着上级的维生素C和总酚类化合物水平（分别为66.49%，645.6 mg·100 g−1 FW和23.9 mg GAE·g−1）。相反，在开花和成熟果期，R. raetam（Forssk.）Webb的脯氨酸（25.4 μmol·g-1 DW）和类胡萝卜素（27.2 μg·g-1 FW）含量显著高于对照。所收集的关于抗氧化活性的数据突出了营养阶段与其他时期相比的有效性。实际上，从植物营养期的芽中获得的水甲醇提取物的IC 50和EC 50值分别为23、380、410、1160和960 μg·mL-1（DPPH·和ABTS·+自由基清除活性、还原力、螯合力和β-胡萝卜素漂白活性）。此外，研究的四个阶段对人体病原体表现出明显的抗菌能力，营养阶段提取物的效率更高。最后，LC-DAD-ESI/MSn分析揭示了作为酚类化合物的主要类别的多酚类化合物的优势，并表明，作为植物生长的函数，个体酚类化合物的生物合成明显不同。这些发现表明，达到最佳效率的R。raetam（Forssk.）韦伯与生理阶段密切相关。
This work focuses on the variability of Retama raetam (Forssk.) Webb bioactive compounds as a function of the plant cycle. The main results showed that it exhibited the highest percentage of polyunsaturated fatty acids, along with superior levels of vitamin C and total phenolic compounds (66.49%, 645.6 mg·100 g−1 FW and 23.9 mg GAE·g−1, respectively) at the vegetative stage. Instead, at the flowering and mature fruiting stages, R. raetam (Forssk.) Webb exhibited notable contents of proline (25.4 μmol·g−1 DW) and carotenoids (27.2 μg·g−1 FW), respectively. The gathered data concerning the antioxidant activity highlighted the effectiveness of the vegetative stage in comparison to the other periods. Actually, IC50 and EC50 values of the hydromethanolic extract obtained from the plant shoots at the vegetative stage were of 23, 380, 410, 1160 and 960 μg·mL−1 (DPPH• and ABTS•+ radicals scavenging activity, reducing power, chelating power and β-carotene bleaching activity, respectively). Furthermore, the four studied stages showed appreciable antibacterial capacities against human pathogens with a higher efficiency of the vegetative stage extract. Finally, the LC-DAD-ESI/MSn analysis revealed the predominance of isoflavonoids as main class of phenolic compounds and demonstrates that individual phenolic biosynthesis was clearly different as a function of plant growth. These findings highlight that reaching the optimum efficiency of R. raetam (Forssk.) Webb is closely linked to the physiological stage.
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