Black-pigmenting gram-negative bacteria in periodontal disease. II. Screening strategies for detection of P. gingivalis.
Black-pigmenting gram-negative bacteria in periodontal disease. II. Screening strategies for detection of P. gingivalis.
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牙周病中的黑色素革兰氏阴性细菌。
DOI：
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发表时间：
1991
影响因子：
3.5
通讯作者：
N. Lang
中科院分区：
文献类型：
--
作者：
A. Mombelli;Hal McNabb;N. Lang
本分析的目的是评估使用选定的地点检测牙龈假单胞菌的可行性，并指出这种有机体在牙周炎患者中的比例增加。在10名患有中度至晚期牙周病的患者中，从每颗牙齿的中、颊、远端和口腔（舌或腭）方面分别采集微生物样本。这总共产生了927个微生物样本，每个患者84到102个。发现三种不同的分布模式和相对比例的牙龈假单胞菌。在一组患者中，没有培养细菌。在第二组中，很少有阳性部位存在低比例的牙龈假单胞菌。第三组患者产生了高频率和比例的牙龈卟啉卟啉。在95%的置信水平上诊断牙龈卟啉卟啉菌存在所需的样本数量在三组之间差异很大。在4例患者中，随机抽取4个样本就足够了，而在其余3例阳性患者中，需要25个或更多的样本才能同样确定地检测到生物体。研究了7种不同的龈下多次采样方案。考虑到检测牙龈卟啉单胞菌存在所需的样本数量和估计该生物的最高比例时，选择每个象限中最深的口袋是最有效的采样方法。
The purpose of this analysis was to evaluate the feasibility of detecting P. gingivalis using selected sites and to indicate increased proportions of this organism in periodontitis patients. In 10 patients suffering from moderate to advanced periodontal disease, separate microbiological samples were taken from the mesial, buccal, distal and oral (lingual or palatal) aspects of every tooth. This yielded a total of 927 microbiological samples, 84 to 102 per patient. Three distinct patterns of distribution and relative proportion of P. gingivalis were recognized. In one group of patients, the organism was not cultured. In a second group, few positive sites with low proportions of P. gingivalis were present. A third group of patients yielded high frequencies and proportions of P. gingivalis. The number of samples necessary to diagnose the presence of P. gingivalis at a 95% confidence level varied considerably between the three groups. In 4 patients, sampling 4 randomly selected sites was sufficient, while in the remaining 3 positive patients, 25 or more samples were required to detect the organism with equal certainty. Seven different protocols for multiple subgingival sampling were studied. When considering the number of samples needed to detect the presence of P. gingivalis and to estimate the highest proportion of this organism, selection of the deepest pocket in each quadrant was the most efficient method of sampling.
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影响因子：
--
作者：
Genco,RJ;Zambon,JJ;Christersson,LA
通讯作者：
Christersson,LA
成人牙周炎治疗后某些龈下细菌与牙周袋深度和牙周附着获得或丧失的关系。
影响因子：
6.7
作者：
Slots,J;Emrich,LJ;Genco,RJ;Rosling,BG
通讯作者：
Rosling,BG
成人早期牙周炎病变的微生物群。
影响因子：
6.7
作者：
Tanner,A;Bouldin,H
通讯作者：
Bouldin,H
某些龈下微生物物种在选定牙周条件下的分布。
影响因子：
3.5
作者：
Savitt,ED;Socransky,SS
通讯作者：
Socransky,SS
在寻找破坏性牙周病的病因中遇到了困难。
影响因子：
6.7
作者：
Socransky,SS;Haffajee,AD;Smith,GL;Dzink,JL
通讯作者：
Dzink,JL