Specific gene-regulation networks during the pre-implantation development of the pig embryo as revealed by deep sequencing.
Specific gene-regulation networks during the pre-implantation development of the pig embryo as revealed by deep sequencing.
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DOI：
10.1186/1471-2164-15-4
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发表时间：
2014-01-03
期刊：
影响因子：
4.4
通讯作者：
Li N
中科院分区：
文献类型：
--
作者：
Cao S;Han J;Wu J;Li Q;Liu S;Zhang W;Pei Y;Ruan X;Liu Z;Wang X;Lim B;Li N
由于很少有研究描述猪植入前胚胎发育（PED）背后独特的分子网络调控，因此猪胚胎的基因工程受到限制。此外，这种研究的缺乏阻碍了猪胚胎干细胞和猪诱导多能干细胞（iPSC）的衍生和应用。我们使用mRNA深度测序鉴定并分析了猪体内衍生和体细胞核移植（SCNT）以及小鼠体内衍生的不同阶段的植入前胚胎的全基因组转录组。猪胚胎转录组与小鼠和人类植入前胚胎的转录组的比较揭示了猪PED期间独特的基因表达模式。通过全基因组基因表达分析，证实猪合子基因组激活发生在4细胞期。在SCNT胚胎中，这种激活被延迟到8细胞期。内细胞团（ICM）和滋养外胚层（TE）的特异性基因表达分析表明，猪和小鼠植入前胚胎在第一谱系分离和原始内胚层分化过程中共享调控网络，但在外胚层定型过程中不共享。此外，脂肪酸代谢似乎是猪植入前胚胎发育的独特特征。此外，在猪SCNT胚胎的整体基因表达模式是不同的，在体内衍生的猪胚胎。我们的研究结果为多能干细胞工程和了解猪的发育提供了资源。本文的在线版本（doi：10.1186/1471-2164-15-4）包含补充材料，可供授权用户使用。
Because few studies exist to describe the unique molecular network regulation behind pig pre-implantation embryonic development (PED), genetic engineering in the pig embryo is limited. Also, this lack of research has hindered derivation and application of porcine embryonic stem cells and porcine induced pluripotent stem cells (iPSCs). We identified and analyzed the genome wide transcriptomes of pig in vivo-derived and somatic cell nuclear transferred (SCNT) as well as mouse in vivo-derived pre-implantation embryos at different stages using mRNA deep sequencing. Comparison of the pig embryonic transcriptomes with those of mouse and human pre-implantation embryos revealed unique gene expression patterns during pig PED. Pig zygotic genome activation was confirmed to occur at the 4-cell stage via genome-wide gene expression analysis. This activation was delayed to the 8-cell stage in SCNT embryos. Specific gene expression analysis of the putative inner cell mass (ICM) and the trophectoderm (TE) revealed that pig and mouse pre-implantation embryos share regulatory networks during the first lineage segregation and primitive endoderm differentiation, but not during ectoderm commitment. Also, fatty acid metabolism appears to be a unique characteristic of pig pre-implantation embryonic development. In addition, the global gene expression patterns in the pig SCNT embryos were different from those in in vivo-derived pig embryos. Our results provide a resource for pluripotent stem cell engineering and for understanding pig development. The online version of this article (doi:10.1186/1471-2164-15-4) contains supplementary material, which is available to authorized users.
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影响因子：
1.8
作者：
Lorthongpanich, Chanchao;Laowtammathron, Chuti;Parnpai, Rangsun
通讯作者：
Parnpai, Rangsun
DOI：
10.1089/clo.2009.0004
发表时间：
2009-06-01
期刊：
CLONING AND STEM CELLS
影响因子：
--
作者：
Chung, Young;Bishop, Colin E.;Lanza, Robert
通讯作者：
Lanza, Robert
影响因子：
2.5
作者：
Hall, Vanessa J.;Christensen, Josef;Hyttel, Poul
通讯作者：
Hyttel, Poul
影响因子：
64.8
作者：
BRAUDE, P;BOLTON, V;MOORE, S
通讯作者：
MOORE, S
影响因子：
4.8
作者：
Esteban, Miguel A.;Xu, Jianyong;Pei, Duanqing
通讯作者：
Pei, Duanqing