Atrophy Resistant vs. Atrophy Susceptible Skeletal Muscles: "aRaS" as a Novel Experimental Paradigm to Study the Mechanisms of Human Disuse Atrophy.
Atrophy Resistant vs. Atrophy Susceptible Skeletal Muscles: "aRaS" as a Novel Experimental Paradigm to Study the Mechanisms of Human Disuse Atrophy.
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抗萎缩与萎缩易感的骨骼肌：“ Aras”是一种新型的实验范式，以研究人类废物萎缩的机制。
DOI：
10.3389/fphys.2021.653060
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发表时间：
2021
影响因子：
4
通讯作者：
Phillips BE
中科院分区：
文献类型：
--
作者：
Bass JJ;Hardy EJO;Inns TB;Wilkinson DJ;Piasecki M;Morris RH;Spicer A;Sale C;Smith K;Atherton PJ;Phillips BE
废用性萎缩（DA）描述了通过不完全确定的机制导致的不活动引起的骨骼肌损失。一个有趣的观察是，尽管个体肌肉表现出相似的解剖功能/位置，但它们却表现出不同程度的萎缩。我们的目标是开发一种创新的实验范例来研究抗萎缩性胫骨前肌 (TA) 和萎缩易感腓肠肌内侧 (MG) 肌肉 (aRaS)，以期揭示中枢机制。七名健康年轻男性（22 ± 1 岁）接受了 15 天的单侧腿部固定 (ULI)。参与者使用护膝和气靴固定单腿，将腿（膝盖弯曲 75°）和脚踝固定到位。在固定期之前和之后对小腿进行双能 X 射线吸收测定法 (DXA)、MRI 和超声扫描，以确定肌肉质量的变化。开发了固定（双肢）后进行壳状切片和微针 TA/MG 肌肉活检的技术，并进行了初步的纤维分型分析。 TA/MG 肌肉显示出类似的纤维类型分布，主要是 I 型纤维（TA 67 ± 7%，MG 63 ± 5%）。固定 15 天后，MG 肌肉体积 (–2.8 ± 1.4%，p < 0.05) 和肌肉厚度下降 (−12.9 ± 1.6%，p < 0.01)，肌肉体积和厚度变化之间呈正相关 (R2 = 0.31，p = 0.038)。重要的是，TA 肌肉的体积和厚度均保持不变。这种独特的“aRaS”范式的使用提供了一种有效且方便的方法来研究人类不同的 DA 易感性的机制基础，这可能有助于新的机制见解，并通过扩展，减轻人类 DA 期间的骨骼肌萎缩。
Disuse atrophy (DA) describes inactivity-induced skeletal muscle loss, through incompletely defined mechanisms. An intriguing observation is that individual muscles exhibit differing degrees of atrophy, despite exhibiting similar anatomical function/locations. We aimed to develop an innovative experimental paradigm to investigate Atrophy Resistant tibialis anterior (TA) and Atrophy Susceptible medial gastrocnemius (MG) muscles (aRaS) with a future view of uncovering central mechanisms. Seven healthy young men (22 ± 1 year) underwent 15 days unilateral leg immobilisation (ULI). Participants had a single leg immobilised using a knee brace and air-boot to fix the leg (75° knee flexion) and ankle in place. Dual-energy X-ray absorptiometry (DXA), MRI and ultrasound scans of the lower leg were taken before and after the immobilisation period to determine changes in muscle mass. Techniques were developed for conchotome and microneedle TA/MG muscle biopsies following immobilisation (both limbs), and preliminary fibre typing analyses was conducted. TA/MG muscles displayed comparable fibre type distribution of predominantly type I fibres (TA 67 ± 7%, MG 63 ± 5%). Following 15 days immobilisation, MG muscle volume (–2.8 ± 1.4%, p < 0.05) and muscle thickness decreased (−12.9 ± 1.6%, p < 0.01), with a positive correlation between changes in muscle volume and thickness (R2 = 0.31, p = 0.038). Importantly, both TA muscle volume and thickness remained unchanged. The use of this unique “aRaS” paradigm provides an effective and convenient means by which to study the mechanistic basis of divergent DA susceptibility in humans, which may facilitate new mechanistic insights, and by extension, mitigation of skeletal muscle atrophy during human DA.
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DOI：
10.1097/00005768-200010000-00013
发表时间：
2000-10-01
期刊：
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
影响因子：
--
作者：
Akima, H;Kawakami, Y;Fukunaga, T
通讯作者：
Fukunaga, T
影响因子：
3.9
作者：
Dideriksen, K.;Boesen, A. P.;Kjaer, M.
通讯作者：
Kjaer, M.
影响因子：
5.5
作者：
Brocca, Lorenza;Cannavino, Jessica;Pellegrino, Maria Antonietta
通讯作者：
Pellegrino, Maria Antonietta
影响因子：
7.1
作者：
English, Kirk L.;Mettler, Joni A.;Paddon-Jones, Douglas
通讯作者：
Paddon-Jones, Douglas
影响因子：
5.5
作者：
Fitts, R. H.;Trappe, S. W.;Riley, D. A.
通讯作者：
Riley, D. A.